RRC ID 61324
著者 Toriumi T, Takayama N, Murakami M, Sato M, Yuguchi M, Yamazaki Y, Eto K, Otsu M, Nakauchi H, Shirakawa T, Isokawa K, Honda MJ.
タイトル Characterization of mesenchymal progenitor cells in the crown and root pulp of primary teeth.
ジャーナル Biomed Res
Abstract The existence of progenitor/mesenchymal stem cells (MSCs) was demonstrated previously in human primary/deciduous teeth. In this study, we examined dental pulp cells from root portion (root cells) of primary teeth without discernible root resorption and compared them with pulp cells from the crown portion (crown cells). Root cells and crown cells were characterized and compared to each other based on progenitor/MSC characteristics and on their generation efficiency of induced pluripotent stem (iPS) cells. Root cells and crown cells included cells manifesting typical progenitor/MSC properties such as osteogenic and adipogenic differentiation potential and clonogenicity. Interestingly, root cells showed a higher expression level of embryonic stem cell marker, KLF4, than crown cells. Moreover, the number of colony-forming unit-fibroblast and cell proliferation rate were higher for root cells than crown cells, and the efficiency of generating iPS cells from root cells was approximately four times higher than that from crown cells. Taken together, these results suggest that root cells from primary teeth show the MSC-like properties and thus could be a potent alternative source for iPS cell generation and the subsequent transplantation therapy.
巻・号 36(1)
ページ 31-45
公開日 2015-1-1
DOI 10.2220/biomedres.36.31
PMID 25749149
MeSH Adipocytes / cytology Adipocytes / metabolism Biomarkers / metabolism Cell Cycle / genetics Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Differentiation Cell Proliferation Cellular Reprogramming / genetics Dental Pulp / cytology* Dental Pulp / metabolism Fibroblasts / cytology Fibroblasts / metabolism Gene Expression Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / genetics Kruppel-Like Transcription Factors / metabolism Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / metabolism Osteoblasts / cytology Osteoblasts / metabolism Primary Cell Culture Tooth Crown / cytology* Tooth Crown / metabolism Tooth Root / cytology* Tooth Root / metabolism Tooth, Deciduous / cytology* Tooth, Deciduous / metabolism
IF 1.429
リソース情報
ヒト・動物細胞 253G1(HPS0002)