論文 - 詳細
| RRC ID | 61393 |
|---|---|
| 著者 | Davis J, Cook ND, Pither RJ. |
| タイトル | Biologic mechanisms of 89SrCl2 incorporation into type I collagen during bone mineralization. |
| ジャーナル | J Nucl Med |
| Abstract |
UNLABELLED:89SrCl2 is currently used as a palliative treatment for painful osseous metastases associated with an osteoblastic reaction in bone. However, the underlying biologic mechanism by which 89SrCl2 accumulates at these lesions and mediates palliation remains unclear. The aim of this study was therefore to elucidate this mechanism. METHODS:An in vitro cell biologic model, incorporating the MC3T3-E1 murine osteoblast cell line, was established to replicate the process of collagen production and mineralization. Experiments were performed to investigate the cellular association of 89SrCl2 and 45CaCl2 with both MC3T3-E1 cells and the PC-3 human prostate adenocarcinoma cell line. RESULTS:No evidence of intracellular localization of 89SrCl2 or 45CaCl2 was found for either cell line. Localization of radiolabel was seen to be associated with MC3T3-E1 cells but only in cultures that had undergone both differentiation and mineralization. The association of 89SrCl2 was inhibited by the alkaline phosphatase inhibitor levamisole, and extracellular localization of 89SrCl2 was confirmed by microautoradiography. CONCLUSION:89SrCl2 acts as a calcium mimic and, as such, becomes associated with the collagen matrix produced by the MC3T3-E1 cells during collagen mineralization. |
| 巻・号 | 41(1) |
| ページ | 183-8 |
| 公開日 | 2000-1-1 |
| PMID | 10647622 |
| MeSH | Animals Bone Neoplasms / radiotherapy* Bone Neoplasms / secondary Bone and Bones / metabolism* Calcification, Physiologic* Calcium Chloride / pharmacokinetics Calcium Radioisotopes / pharmacokinetics Cell Line Collagen / metabolism* Humans Male Mice Palliative Care Prostatic Neoplasms / pathology Strontium / pharmacokinetics* Strontium / therapeutic use Strontium Radioisotopes / pharmacokinetics* Strontium Radioisotopes / therapeutic use Time Factors Tumor Cells, Cultured |
| IF | 7.887 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |