論文 - 詳細
| RRC ID | 61430 |
|---|---|
| 著者 | Seike S, Miyamoto K, Kobayashi K, Takehara M, Nagahama M. |
| タイトル | Clostridium perfringens Delta-Toxin Induces Rapid Cell Necrosis. |
| ジャーナル | PLoS One |
| Abstract |
Clostridium perfringens delta-toxin is a β-pore-forming toxin and a putative pathogenic agent of C. perfringens types B and C. However, the mechanism of cytotoxicity of delta-toxin remains unclear. Here, we investigated the mechanisms of cell death induced by delta-toxin in five cell lines (A549, A431, MDCK, Vero, and Caco-2). All cell lines were susceptible to delta-toxin. The toxin caused rapid ATP depletion and swelling of the cells. Delta-toxin bound and formed oligomers predominantly in plasma membrane lipid rafts. Destruction of the lipid rafts with methyl β-cyclodextrin inhibited delta-toxin-induced cytotoxicity and ATP depletion. Delta-toxin caused the release of carboxyfluorescein from sphingomyelin-cholesterol liposomes and formed oligomers; toxin binding to the liposomes declined with decreasing cholesterol content in the liposomes. Flow cytometric assays with annexin V and propidium iodide revealed that delta-toxin treatment induced an elevation in the population of annexin V-negative and propidium iodide-positive cells. Delta-toxin did not cause the fragmentation of DNA or caspase-3 activation. Furthermore, delta-toxin caused damage to mitochondrial membrane permeability and cytochrome c release. In the present study, we demonstrate that delta-toxin produces cytotoxic activity through necrosis. |
| 巻・号 | 11(1) |
| ページ | e0147957 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.pone.0147957 |
| PII | PONE-D-15-34941 |
| PMID | 26807591 |
| PMC | PMC4726729 |
| MeSH | Animals Bacterial Toxins* Caco-2 Cells Caspase 3 / metabolism Cell Death / drug effects* Chlorocebus aethiops DNA Fragmentation / drug effects Fluoresceins / metabolism Humans Membrane Microdomains / drug effects Membrane Microdomains / metabolism Necrosis / chemically induced* Necrosis / metabolism Vero Cells |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) A431(RCB0202) CACO-2(RCB0988) Vero(RCB0001) MDCK(RCB0995) |