論文 - 詳細
RRC ID | 61431 |
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著者 | Nagahama M, Ohkubo A, Kinouchi Y, Kobayashi K, Miyamoto K, Takehara M, Sakurai J. |
タイトル | Clostridium perfringens TpeL Induces Formation of Stress Fibers via Activation of RhoA-ROCK Signaling Pathway. |
ジャーナル | Biol Pharm Bull |
Abstract |
Clostridium perfringens TpeL belongs to a family of large clostridial glucosylating cytotoxins. TpeL modifies Rac1 and Ras subfamily proteins. Herein we report TpeL-induced formation of stress fibers via RhoA-Rho kinase (ROCK) signaling. A recombinant protein (TpeL1-525) derived from the TpeL N-terminal catalytic domain in the presence of streptolysin O (SLO) induced the formation of actin stress fibers in Madin-Darby canine kidney (MDCK) cells in a dose-dependent manner. The RhoA/ROCK pathway is known to control the formation of stress fibers. We examined the role of the RhoA/ROCK pathway in TpeL-induced formation of stress fibers. TpeL1-525-induced formation of stress fibers was inhibited by the ROCK inhibitor, Y27632 and Rho protein inhibitor, C3 transferase. TpeL1-525 activated RhoA and ROCK in a dose-dependent manner. C3 transferase blocked TpeL1-525-induced activation of RhoA and ROCK whereas Y27632 inhibited TpeL-induced activation of ROCK. These results demonstrate for the first time that TpeL induces the formation of stress fibers by activating the RhoA/ROCK signaling pathway. |
巻・号 | 38(5) |
ページ | 732-9 |
公開日 | 2015-1-1 |
DOI | 10.1248/bpb.b14-00842 |
PMID | 25947919 |
MeSH | Actins / metabolism* Amides / pharmacology Animals Bacterial Toxins / pharmacology* Clostridium Infections / metabolism* Clostridium perfringens / metabolism Clostridium perfringens / pathogenicity* Dogs Enzyme Inhibitors / pharmacology Madin Darby Canine Kidney Cells Pyridines / pharmacology Signal Transduction Stress Fibers / metabolism* Transferases / pharmacology rac1 GTP-Binding Protein / metabolism ras Proteins / metabolism rho-Associated Kinases / metabolism* rhoA GTP-Binding Protein / metabolism* |
IF | 1.863 |
リソース情報 | |
ヒト・動物細胞 | MDCK(RCB0995) |