RRC ID 61432
著者 Gao F, Wang J, Ji G, Liu S, Yao Y, Wang T, Wu H, Xia Y, Gong D, Jiang H, Yang H, Zhang X.
タイトル Clustering of Cancer Cell Lines Using A Promoter- Targeted Liquid Hybridization Capture-Based Bisulfite Sequencing Approach.
ジャーナル Technol Cancer Res Treat
Abstract DNA methylation plays a significant role in assuring cell identity, thus potentiating its application in molecular classification of cancers in respect to tissue-origins or clinically and etiologically distinct subtypes. In this study, we optimized our liquid hybridization capture-based bisulfite sequencing (LHC-BS) approach on the gene promoter regions of 11 cell lines. Our results indicated that promoter methylomes could not only cluster cancer cell lines with respect to tissue origins but also differentiate cancer subtypes based on CpG island methylator phenotype (CIMP). Promoter-targeted LHC-BS as means for comprehensive screening and classifying cancer cells with promoter methylomes provided a powerful strategy for further complex clinical studies.
巻・号 14(4)
ページ 383-94
公開日 2015-8-1
DOI 10.1177/1533034614500416
PII 14/4/383
PMID 26269607
MeSH Cell Line, Tumor Cluster Analysis* DNA Methylation Gene Expression Regulation, Neoplastic* Humans Neoplasms / genetics* Nucleic Acid Hybridization* Promoter Regions, Genetic* Sequence Analysis, DNA
IF 2.074
リソース情報
ヒト・動物細胞 OS-RC-2(RCB0735)