論文 - 詳細
| RRC ID | 61438 |
|---|---|
| 著者 | Hanks T, Atkinson BL. |
| タイトル | Comparison of cell viability on anorganic bone matrix with or without P-15 cell binding peptide. |
| ジャーナル | Biomaterials |
| Abstract |
Cell-extracellular cell matrix (ECM) interactions play a vital role in development including cytoskeletal structure, growth and differentiation. A 15 amino acid, non-RGD containing, peptide that is derived from type I collagen was tested for its ability to confer cell survival. Two routinely utilized bone graft materials, anorganic bone matrix (ABM) and ABM carrying the cell binding peptide P-15 were compared for cell viability and apoptosis. Anchorage-dependent human foreskin fibroblasts (HFF) or osteogenic MC3T3-E1 cells were seeded on ABM or ABM/P-15. After serum withdrawal, viability and level of apoptosis were significantly (p<0.05) improved for cells on ABM/P-15 compared to cells on ABM. Scanning electron microscopy confirmed large, spread cells on ABM/P-15 and smaller cells with apoptotic cellular blebs on ABM. In addition, viable cell attachment was significantly greater on cells cultured on ABM/P-15 compared with demineralized freeze-dried bone allograft. This is the first report that the P-15 cell binding peptide, when complexed with ABM, promotes cell survival. The P-15 peptide may modulate cell number and tissue structure by the enhancement of viable cell attachment and via the regulation of apoptosis. |
| 巻・号 | 25(19) |
| ページ | 4831-6 |
| 公開日 | 2004-8-1 |
| DOI | 10.1016/j.biomaterials.2003.12.007 |
| PII | S0142961203011529 |
| PMID | 15120530 |
| MeSH | 3T3 Cells Animals Biomimetic Materials / chemistry Biomimetic Materials / pharmacology Bone Substitutes / chemistry* Bone Transplantation / methods Cell Adhesion / drug effects Cell Adhesion / physiology Cell Survival / drug effects Cell Survival / physiology Collagen / chemistry* Collagen / pharmacology* Extracellular Matrix / chemistry* Fibroblasts / cytology* Fibroblasts / drug effects Fibroblasts / physiology* Humans Inorganic Chemicals / chemistry Materials Testing Mice Peptide Fragments / chemistry* Peptide Fragments / pharmacology* Tissue Engineering / methods* |
| IF | 10.317 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 31 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |