論文 - 詳細
| RRC ID | 6144 |
|---|---|
| 著者 | Alvarez C, Calo L, Romero LC, García I, Gotor C. |
| タイトル | An O-acetylserine(thiol)lyase homolog with L-cysteine desulfhydrase activity regulates cysteine homeostasis in Arabidopsis. |
| ジャーナル | Plant Physiol |
| Abstract |
Cysteine (Cys) occupies a central position in plant metabolism due to its biochemical functions. Arabidopsis (Arabidopsis thaliana) cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes that catalyze the biosynthesis of Cys. Because they are localized in the cytosol, plastids, and mitochondria, this results in multiple subcellular Cys pools. Much progress has been made on the most abundant OASTL enzymes; however, information on the less abundant OASTL-like proteins has been scarce. To unequivocally establish the enzymatic reaction catalyzed by the minor cytosolic OASTL isoform CS-LIKE (for Cys synthase-like; At5g28030), we expressed this enzyme in bacteria and characterized the purified recombinant protein. Our results demonstrate that CS-LIKE catalyzes the desulfuration of L-Cys to sulfide plus ammonia and pyruvate. Thus, CS-LIKE is a novel L-Cys desulfhydrase (EC 4.4.1.1), and we propose to designate it DES1. The impact and functionality of DES1 in Cys metabolism was revealed by the phenotype of the T-DNA insertion mutants des1-1 and des1-2. Mutation of the DES1 gene leads to premature leaf senescence, as demonstrated by the increased expression of senescence-associated genes and transcription factors. Also, the absence of DES1 significantly reduces the total Cys desulfuration activity in leaves, and there is a concomitant increase in the total Cys content. As a consequence, the expression levels of sulfur-responsive genes are deregulated, and the mutant plants show enhanced antioxidant defenses and tolerance to conditions that promote oxidative stress. Our results suggest that DES1 from Arabidopsis is an L-Cys desulfhydrase involved in maintaining Cys homeostasis, mainly at late developmental stages or under environmental perturbations. |
| 巻・号 | 152(2) |
| ページ | 656-69 |
| 公開日 | 2010-2-1 |
| DOI | 10.1104/pp.109.147975 |
| PII | pp.109.147975 |
| PMID | 19955263 |
| PMC | PMC2815857 |
| MeSH | Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Carbon-Oxygen Lyases / genetics Carbon-Oxygen Lyases / metabolism* Cloning, Molecular Cystathionine gamma-Lyase / genetics Cystathionine gamma-Lyase / metabolism* Cysteine / metabolism* DNA, Bacterial / genetics Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Regulation, Plant Glutathione / metabolism Homeostasis Mutagenesis, Insertional Oxidative Stress Plant Leaves / genetics Plant Leaves / metabolism RNA, Plant / genetics Sulfates / metabolism |
| IF | 6.902 |
| 引用数 | 187 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst16025 |