論文 - 詳細
| RRC ID | 61453 |
|---|---|
| 著者 | Kakizawa S, Kishimoto Y, Yamamoto S, Onga K, Yasuda K, Miyamoto Y, Watanabe M, Sakai R, Mori N. |
| タイトル | Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. |
| ジャーナル | Sci Rep |
| Abstract |
Intracellular Ca2+ levels are changed by influx from extracellular medium and release from intracellular stores. In the central nervous systems, Ca2+ release is involved in various physiological events, such as neuronal excitability and transmitter release. Although stable Ca2+ release in response to stimulus is critical for proper functions of the nervous systems, regulatory mechanisms relating to Ca2+ release are not fully understood in central neurons. Here, we demonstrate that ShcB, an adaptor protein expressed in central neurons, has an essential role in functional maintenance of Ca2+ store in cerebellar Purkinje cells (PCs). ShcB-knockout (KO) mice showed defects in cerebellar-dependent motor function and long-term depression (LTD) at cerebellar synapse. The reduced LTD was accompanied with an impairment of intracellular Ca2+ release. Although the expression of Ca2+ release channels and morphology of Ca2+ store looked intact, content of intracellular Ca2+ store and activity of sarco/endoplasmic reticular Ca2+-ATPase (SERCA) were largely decreased in the ShcB-deficient cerebellum. Furthermore, when ShcB was ectopically expressed in the ShcB-KO PCs, the Ca2+ release and its SERCA-dependent component were restored. These data indicate that ShcB plays a key role in the functional maintenance of ER Ca2+ store in central neurons through regulation of SERCA activity. |
| 巻・号 | 10(1) |
| ページ | 14475 |
| 公開日 | 2020-9-2 |
| DOI | 10.1038/s41598-020-71414-y |
| PII | 10.1038/s41598-020-71414-y |
| PMID | 32879382 |
| PMC | PMC7468156 |
| MeSH | Animals Calcium / metabolism Calcium Signaling / genetics Cerebellum / metabolism* Cerebellum / pathology Endoplasmic Reticulum / genetics Humans Long-Term Synaptic Depression / genetics* Mice Mice, Knockout Motor Disorders / genetics Motor Disorders / physiopathology Neuronal Plasticity / genetics Purkinje Cells / metabolism Purkinje Cells / pathology Sarcoplasmic Reticulum Calcium-Transporting ATPases / genetics* Src Homology 2 Domain-Containing, Transforming Protein 2 / genetics* Synapses / genetics* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-MCS-IRES2-mRFP1 (RDB07918) CSII-CMV-mRFP1 (RDB06386) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |
| ヒト・動物細胞 | 293T(RCB2202) |