論文 - 詳細
| RRC ID | 61468 |
|---|---|
| 著者 | Koyama S, Narita E, Shinohara N, Miyakoshi J. |
| タイトル | Effect of low-dose X-ray irradiation on micronucleus formation in human embryo, newborn and child cells. |
| ジャーナル | Int J Radiat Biol |
| Abstract |
PURPOSE:It is well known that a high-dose of ionizing radiation is sufficient to break DNA strands, which leads to elevated genotoxic risks; however, the risks associated with low doses of ionizing radiation remain unclear. In addition, there is little data about the effect of low-dose ionizing radiation on human-derived embryo, newborn and child cells. We investigated the frequency of micronucleus (MN) formation in these cells to understand the genotoxic effects of ionizing radiation. MATERIALS AND METHODS:We irradiated the cells with X-rays from 0.02-2 Gy at a rate of 0.0635 Gy/min. After irradiation, we investigated the effect of low-dose X-ray irradiation on cellular viability and frequency of MN formation. RESULTS:Increases in MN formation were largely dose-dependent; however, there were no differences between controls and doses lower than 0.2 Gy, except in KMST-6 human transformed embryo cells. CONCLUSION:We could not detect an obvious effect of low-dose X-ray irradiation at doses lower than 0.1 Gy. The embryonic cells were more sensitive to X-ray irradiation than newborn and child cells. The threshold for X-ray-induced MN formation appears to be in the range of 0.05-0.1 Gy in cultured human embryo, newborn and child cells. |
| 巻・号 | 92(12) |
| ページ | 790-795 |
| 公開日 | 2016-12-1 |
| DOI | 10.1080/09553002.2016.1221544 |
| PMID | 27579525 |
| MeSH | Aging / radiation effects* Cell Survival / genetics Cell Survival / radiation effects* Child Dose-Response Relationship, Radiation Embryo, Mammalian / cytology* Embryo, Mammalian / radiation effects* Female Humans Infant, Newborn Male Micronuclei, Chromosome-Defective / radiation effects* Radiation Dosage X-Rays / adverse effects* |
| IF | 2.368 |
| リソース情報 | |
| ヒト・動物細胞 | KMST-6(RCB1955) |