RRC ID 61473
Author Mukai R, Kawabata K, Otsuka S, Ishisaka A, Kawai Y, Ji ZS, Tsuboi H, Terao J.
Title Effect of quercetin and its glucuronide metabolite upon 6-hydroxydopamine-induced oxidative damage in Neuro-2a cells.
Journal Free Radic Res
Abstract Quercetin is ubiquitously distributed in plant foods. This antioxidative polyphenol is mostly converted to conjugated metabolites in the body. Parkinson disease (PD) has been suggested to be related to oxidative stress derived from abnormal dopaminergic activity. We evaluated if dietary quercetin contributes to the antioxidant network in the central nervous system from the viewpoint of PD prevention. A neurotoxin, 6-hydroxydopamine (6-OHDA), was used as a model of PD. 6-OHDA-induced H₂O₂ production and cell death in mouse neuroblastoma, Neuro-2a. Quercetin aglycone suppressed 6-OHDA-induced H₂O₂ production and cell death, although aglycone itself reduced cell viability at higher concentration. Quercetin 3-O-β-D-glucuronide (Q3GA), which is an antioxidative metabolite of dietary quercetin, was little incorporated into the cell resulting in neither suppression of 6-OHDA-induced cell death nor reduction of cell viability. Q3GA was found to be deconjugated to quercetin by microglial MG-6 cells. These results indicate that quercetin metabolites should be converted to their aglycone to exert preventive effect on damage to neuronal cells.
Volume 46(8)
Pages 1019-28
Published 2012-8-1
DOI 10.3109/10715762.2012.673720
PMID 22404304
MeSH Animals Antioxidants / pharmacology* Biological Transport Cell Death Cell Line, Tumor Cell Survival Dose-Response Relationship, Drug Hydrogen Peroxide / metabolism Mice Neurons / cytology Neurons / drug effects* Neurons / metabolism Oxidation-Reduction Oxidative Stress Oxidopamine / pharmacology* Quercetin / analogs & derivatives* Quercetin / metabolism Quercetin / pharmacology
IF 2.839
Human and Animal Cells MG6(RCB2403)