Reference - Detail
| RRC ID | 61474 |
|---|---|
| Author | Nishimura K, Matsumoto R, Yonezawa Y, Nakagawa H. |
| Title | Effect of quercetin on cell protection via erythropoietin and cell injury of HepG2 cells. |
| Journal | Arch Biochem Biophys |
| Abstract |
Quercetin is a flavonoid that has roles in both cytoprotection and cytotoxicity. The relation of queretin's cytoprotective and cytotoxic effects are unknown. Quercetin has been shown to induce expression of hypoxia-inducible factor, a protein that is known to regulate transcription of the erythropoietin (EPO) gene, and EPO is known to have a cytoprotective effect. This study used HepG2 cells to assess whether the cell-protective and/or cytotoxic roles of quercetin are mediated by promotion of EPO production. Increases in the levels of HIF-1α protein and EPO mRNA were quercetin concentration-dependent, with significant increases observed from 10 μM quercetin. Silencing of EPO expression by si-EPO RNA attenuated quercetin-induced cytoprotection against hydrogen peroxide toxicity. Cytotoxicity, evidenced by the induction of apoptosis, was significantly increased by exposure to 50 μM quercetin. Specifically, the levels of cleaved caspase-3 and Bax and the rate of cell death increased, and the level of Bcl-2 decreased, in cells treated with 50 μM quercetin. In contrast, exposure to 10 μM quercetin attenuated cisplatin-induced apoptosis. However, quercetin's ability to protect cells from cisplatin-induced apoptosis was eliminated when EPO expression was silenced using si-EPO RNA. Together, these results suggested that quercetin's cytoprotective effects in HepG2 cells are mediated via EPO production. |
| Volume | 636 |
| Pages | 11-16 |
| Published | 2017-12-15 |
| DOI | 10.1016/j.abb.2017.10.013 |
| PII | S0003-9861(17)30611-2 |
| PMID | 29080630 |
| MeSH | Caspase 3 / biosynthesis Cytoprotection / drug effects* Cytotoxins / pharmacology Erythropoietin / biosynthesis* Hep G2 Cells Humans Hydrogen Peroxide / metabolism* Proto-Oncogene Proteins c-bcl-2 / biosynthesis Quercetin / pharmacology* bcl-2-Associated X Protein / biosynthesis |
| IF | 3.391 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2 |