論文 - 詳細
| RRC ID | 6149 |
|---|---|
| 著者 | Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inzé D, Berx G, De Veylder L. |
| タイトル | The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. |
| ジャーナル | PLoS Genet |
| Abstract |
The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened the impact on plant growth of the replication stress caused by ETG1 deficiency because of inefficient DNA repair. We conclude that the ETG1 replication factor is required for efficient cohesion and that cohesion establishment is essential for proper development of plants suffering from endogenous DNA stress. Cohesion defects observed upon knockdown of its human counterpart suggest an equally important developmental role for the orthologous mammalian ETG1 protein. |
| 巻・号 | 6(1) |
| ページ | e1000817 |
| 公開日 | 2010-1-15 |
| DOI | 10.1371/journal.pgen.1000817 |
| PMID | 20090939 |
| PMC | PMC2806904 |
| MeSH | Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Arabidopsis / chemistry Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Line DNA Repair* Humans Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism* Sequence Alignment Sister Chromatid Exchange* |
| IF | 5.175 |
| 引用数 | 46 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | psh15248 |