論文 - 詳細
| RRC ID | 61510 |
|---|---|
| 著者 | Tsuboi K, Tsuchida Y, Nose T, Ando K. |
| タイトル | Cytotoxic effect of accelerated carbon beams on glioblastoma cell lines with p53 mutation: clonogenic survival and cell-cycle analysis. |
| ジャーナル | Int J Radiat Biol |
| Abstract |
PURPOSE:The cytotoxic effect of high-LET carbon beams was analysed on p53 mutant and wild-type glioblastoma cell lines. MATERIALS AND METHODS:Three glioblastoma (U251MG, TK-1, A-172) cell lines, one medulloblastoma (ONS-76) and one fibroblast cell line (NB1RGB) were used. U251MG and TK-1 have mutated p53, A-172, ONS-76 and NB1RGB have wild-type p53. Gamma-ray and 290 MeV/u carbon mono-peak beams with average LET values of 20, 40, 81 and 105keV/microm were used. Cytotoxicity was measured by clonogenic survival assay and DNA histograms were analysed by flow cytometry. RESULTS:The RBE values for carbon beams at D10 ranged from approximately 1.5-1.7 (20keV) to 2.9-3.1 (105keV). Although p53 mutants were more resistant than wild-types for all radiation qualities, carbon beams yielded a higher RBE in p53 mutants than in wild-types. Alpha values were significantly smaller in p53 mutants than wild-types for gamma- and 20 keV/microm radiations, while no significant difference was noticed for LET greater than 40 keV/microm. A G1 block was noticed after irradiation with gamma-rays and carbon beams of 20 and 40keV/microm in p53 wild-types. A more pronounced G2 block occurred in p53 mutants than wild-types in proportion to LET up to 105 keV/microm. CONCLUSION:Accelerated carbon beams can yield higher RBE in gamma-resistant glioblastoma cell lines with p53 mutations. High-LET irradiation induces not only disappearance of the p53-dependent G1 block but also a greater G2 block in glioblastoma cell lines. |
| 巻・号 | 74(1) |
| ページ | 71-9 |
| 公開日 | 1998-7-1 |
| DOI | 10.1080/095530098141744 |
| PMID | 9687977 |
| MeSH | Cell Cycle / radiation effects* Cell Survival / radiation effects* Cesium Radioisotopes / adverse effects Clone Cells / radiation effects DNA / analysis Dose-Response Relationship, Radiation Flow Cytometry Gamma Rays / adverse effects* Genes, p53 / radiation effects* Glioblastoma / metabolism* Humans Linear Energy Transfer / physiology Radiation, Ionizing Relative Biological Effectiveness Tumor Cells, Cultured / radiation effects* |
| IF | 2.368 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |