RRC ID 61510
Author Tsuboi K, Tsuchida Y, Nose T, Ando K.
Title Cytotoxic effect of accelerated carbon beams on glioblastoma cell lines with p53 mutation: clonogenic survival and cell-cycle analysis.
Journal Int J Radiat Biol
Abstract PURPOSE:The cytotoxic effect of high-LET carbon beams was analysed on p53 mutant and wild-type glioblastoma cell lines.
MATERIALS AND METHODS:Three glioblastoma (U251MG, TK-1, A-172) cell lines, one medulloblastoma (ONS-76) and one fibroblast cell line (NB1RGB) were used. U251MG and TK-1 have mutated p53, A-172, ONS-76 and NB1RGB have wild-type p53. Gamma-ray and 290 MeV/u carbon mono-peak beams with average LET values of 20, 40, 81 and 105keV/microm were used. Cytotoxicity was measured by clonogenic survival assay and DNA histograms were analysed by flow cytometry.
RESULTS:The RBE values for carbon beams at D10 ranged from approximately 1.5-1.7 (20keV) to 2.9-3.1 (105keV). Although p53 mutants were more resistant than wild-types for all radiation qualities, carbon beams yielded a higher RBE in p53 mutants than in wild-types. Alpha values were significantly smaller in p53 mutants than wild-types for gamma- and 20 keV/microm radiations, while no significant difference was noticed for LET greater than 40 keV/microm. A G1 block was noticed after irradiation with gamma-rays and carbon beams of 20 and 40keV/microm in p53 wild-types. A more pronounced G2 block occurred in p53 mutants than wild-types in proportion to LET up to 105 keV/microm.
CONCLUSION:Accelerated carbon beams can yield higher RBE in gamma-resistant glioblastoma cell lines with p53 mutations. High-LET irradiation induces not only disappearance of the p53-dependent G1 block but also a greater G2 block in glioblastoma cell lines.
Volume 74(1)
Pages 71-9
Published 1998-7-1
DOI 10.1080/095530098141744
PMID 9687977
MeSH Cell Cycle / radiation effects* Cell Survival / radiation effects* Cesium Radioisotopes / adverse effects Clone Cells / radiation effects DNA / analysis Dose-Response Relationship, Radiation Flow Cytometry Gamma Rays / adverse effects* Genes, p53 / radiation effects* Glioblastoma / metabolism* Humans Linear Energy Transfer / physiology Radiation, Ionizing Relative Biological Effectiveness Tumor Cells, Cultured / radiation effects*
IF 2.368
Human and Animal Cells NB1RGB(RCB0222)