| RRC ID |
6151
|
| Author |
Chikayama E, Sekiyama Y, Okamoto M, Nakanishi Y, Tsuboi Y, Akiyama K, Saito K, Shinozaki K, Kikuchi J.
|
| Title |
Statistical indices for simultaneous large-scale metabolite detections for a single NMR spectrum.
|
| Journal |
Anal Chem
|
| Abstract |
NMR-based metabolomics has become a practical and analytical methodology for discovering novel genes, biomarkers, metabolic phenotypes, and dynamic cell behaviors in organisms. Recent developments in NMR-based metabolomics, however, have not concentrated on improvements of comprehensiveness in terms of simultaneous large-scale metabolite detections. To resolve this, we have devised and implemented a statistical index, the SpinAssign p-value, in NMR-based metabolomics for large-scale metabolite annotation and publicized this information. It enables simultaneous annotation of more than 200 candidate metabolites from the single (13)C-HSQC (heteronuclear single quantum coherence) NMR spectrum of a single sample of cell extract.
|
| Volume |
82(5)
|
| Pages |
1653-8
|
| Published |
2010-3-1
|
| DOI |
10.1021/ac9022023
|
| PMID |
20128615
|
| MeSH |
Metabolomics*
Nuclear Magnetic Resonance, Biomolecular / methods*
|
| IF |
6.785
|
| Times Cited |
89
|
|
WOS Category
|
CHEMISTRY, ANALYTICAL
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00008 |
