論文 - 詳細
| RRC ID | 61548 |
|---|---|
| 著者 | Zhou X, Tai A, Yamamoto I. |
| タイトル | Enhancement of neurite outgrowth in PC12 cells stimulated with cyclic AMP and NGF by 6-acylated ascorbic acid 2-O-alpha-glucosides (6-Acyl-AA-2G), novel lipophilic ascorbate derivatives. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
It has been shown that ascorbate (AsA) and its stable derivative, ascorbic acid 2-O-alpha-glucoside (AA-2G), do not elicit neurite outgrowth in PC12 cells. However, these ascorbates are synergistically enhanced by both dibutyryl cyclic AMP (Bt(2)cAMP)- and nerve growth factor (NGF)-induced neurite outgrowth in this model. In the present study, the effects of a series of novel lipophilic ascorbate derivatives, 6-acylated ascorbic acid 2-O-alpha-glucosides (6-Acyl-AA-2G), on neurite outgrowth induced by Bt(2)cAMP and NGF were examined in PC12 cells. We found that all the tested acylated ascorbate derivatives enhanced neurite formation induced by both agents in a dose-dependent manner. Of the 6-Acyl-AA-2G derivatives, 6-octanoyl ascorbic acid 2-O-alpha-glucoside (6-Octa-AA-2G) enhanced the Bt(2)cAMP-induced phosphorylated MAPK p44 and p42 expression. A alpha-glucosidase inhibitor, castanospermine, completely abrogated the promotion of neurite outgrowth and MAPK expression by 6-Octa-AA-2G. Addition of 6-Octa-AA-2G (0.5 mM) to PC12 cells caused a rapid and significant increase in intracellular AsA content, which reached a maximum and was maintained from 12 to 24 h after the culture. These findings suggest that 6-Acyl-AA-2G is rapidly hydrolyzed to AsA within the cell and enhances neurite differentiation through the interaction with the inducer-activated MAPK pathway. |
| 巻・号 | 26(3) |
| ページ | 341-6 |
| 公開日 | 2003-3-1 |
| DOI | 10.1248/bpb.26.341 |
| PMID | 12612444 |
| MeSH | Acylation Animals Ascorbic Acid / analogs & derivatives* Ascorbic Acid / chemistry Ascorbic Acid / pharmacology* Blotting, Western Cell Count Cell Line Cyclic AMP / pharmacology* Dose-Response Relationship, Drug Drug Interactions Enzyme Inhibitors / pharmacology Extracellular Space / drug effects Extracellular Space / metabolism Indolizines / pharmacology Mitogen-Activated Protein Kinases / metabolism Nerve Growth Factor / pharmacology* Neurites / drug effects* Neurites / physiology PC12 Cells / drug effects* PC12 Cells / metabolism PC12 Cells / physiology Rats |
| IF | 1.863 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |