RRC ID 61551
Author Wu AT, Aoki T, Sakoda M, Ohta S, Ichimura S, Ito T, Ushida T, Furukawa KS.
Title Enhancing osteogenic differentiation of MC3T3-E1 cells by immobilizing inorganic polyphosphate onto hyaluronic acid hydrogel.
Journal Biomacromolecules
Abstract In tissue engineering, precise control of cues in the microenvironment is essential to stimulate cells to undergo bioactivities such as proliferation, differentiation, and matrix production. However, current approaches are inefficient in providing nondepleting cues. In this study, we have developed a novel bioactive hydrogel (HAX-PolyP) capable of enhancing tissue growth by conjugating inorganic polyphosphate chains onto hyaluronic acid macromers. The immobilized polyphosphates provided constant osteoconductive stimulation to the embedded murine osteoblast precursor cells, resulting in up-regulation of osteogenic marker genes and enhanced levels of ALP activity. The osteoconductive activity was significantly higher when compared to those stimulated with free-floating polyphosphates. Even at very low concentrations, immobilization of polyphosphates onto the scaffold allowed sufficient signaling leading to more effective osteoconduction. These results demonstrate the potential of our novel material as an injectable bioactive scaffold, which can be clinically useful for developing bone grafts and bone regeneration applications.
Volume 16(1)
Pages 166-73
Published 2015-1-12
DOI 10.1021/bm501356c
PMID 25423088
MeSH Animals Cell Culture Techniques / methods Cell Differentiation / drug effects* Cell Differentiation / physiology Hyaluronic Acid / chemistry* Hyaluronic Acid / pharmacology Hydrogels / chemistry* Hydrogels / pharmacology Mice Osteoblasts / drug effects Osteoblasts / physiology Osteogenesis / drug effects* Osteogenesis / physiology Polyphosphates / chemistry* Polyphosphates / pharmacology Tissue Engineering / methods
Human and Animal Cells MC3T3-E1(RCB1126)