Reference - Detail
| RRC ID | 61562 |
|---|---|
| Author | Imano M, Itoh T, Satou T, Kido A, Tsubaki M, Yasuda A, Kato H, Imamoto H, Nishida S, Furukawa H, Takeyama Y, Okuno K, Shiozaki H. |
| Title | Establishment of a novel model of peritoneal carcinomatosis of the peritoneal extension type. |
| Journal | Anticancer Res |
| Abstract |
AIM:Patients with scirrhous carcinoma of the gastrointestinal tract frequently develop peritoneal carcinomatosis-particularly of the peritoneal extension type (PET), which has a bad prognosis. We developed a novel animal model, suitable for testing treatments for PET. MATERIAL AND METHODS:In order to develop the model, we scraped the entire peritoneum of Fischer 344 rats with sterile cotton swabs and injected 1 × 10(6) cells of the RCN-9 cell type into the peritoneal cavity. RESULTS:In the novel experimental model, RCN-9 cells adhered only to the exposed basement membrane. The submesothelial layer and fibroblasts in the submesothelial layer grew and increased to a maximum at day 7, then decreased during late-phase peritoneal carcinomatosis. At day 14, RCN-9 cells coated the peritoneum in a manner similar to PET. CONCLUSION:We successfully established a novel animal model of peritoneal carcinomatosis that mimics clinicopathological features of PET. Fibroblasts in the submesothelial layer potentially play an important role in peritoneal carcinomatosis. |
| Volume | 33(4) |
| Pages | 1439-46 |
| Published | 2013-4-1 |
| PII | 33/4/1439 |
| PMID | 23564784 |
| MeSH | Animals Cell Transformation, Neoplastic* Colonic Neoplasms / pathology* Disease Models, Animal* Male Peritoneal Neoplasms / secondary* Rats Rats, Inbred F344 Tumor Cells, Cultured |
| IF | 1.994 |
| Resource | |
| Human and Animal Cells | RCN-9(RCB0511) |