論文 - 詳細
| RRC ID | 61586 |
|---|---|
| 著者 | Tashiro K, Hirata N, Okada A, Yamaguchi T, Takayama K, Mizuguchi H, Kawabata K. |
| タイトル | Expression of coxsackievirus and adenovirus receptor separates hematopoietic and cardiac progenitor cells in fetal liver kinase 1-expressing mesoderm. |
| ジャーナル | Stem Cells Transl Med |
| Abstract |
In developing embryos or in vitro differentiation cultures using pluripotent stem cells (PSCs), such as embryonic stem cells and induced pluripotent stem cells, fetal liver kinase 1 (Flk1)-expressing mesodermal cells are thought to be a heterogeneous population that includes hematopoietic progenitors, endothelial progenitors, and cardiac progenitors. However, information on cell surface markers for separating these progenitors in Flk1⁺ cells is currently limited. In the present study, we show that distinct types of progenitor cells in Flk1⁺ cells could be separated according to the expression of coxsackievirus and adenovirus receptor (CAR, also known as CXADR), a tight junction component molecule. We found that mouse and human PSC- and mouse embryo-derived Flk1⁺ cells could be subdivided into Flk1⁺CAR⁺ cells and Flk1⁺CAR⁻ cells. The progenitor cells with cardiac potential were almost entirely restricted to Flk1⁺CAR⁺ cells, and Flk1⁺CAR⁻ cells efficiently differentiated into hematopoietic cells. Endothelial differentiation potential was observed in both populations. Furthermore, from the expression of CAR, Flk1, and platelet-derived growth factor receptor-α (PDGFRα), Flk1⁺ cells could be separated into three populations (Flk1⁺PDGFRα⁻ CAR⁻ cells, Flk1⁺PDGFRα⁻CAR⁺ cells, and Flk1⁺PDGFRα⁺CAR⁺ cells). Flk1⁺PDGFRα⁺ cells and Flk1⁺PDGFRα⁻ cells have been reported as cardiac and hematopoietic progenitor cells, respectively. We identified a novel population (Flk1⁺PDGFRα⁻ CAR⁺ cells) with the potential to differentiate into not only hematopoietic cells and endothelial cells but also cardiomyocytes. Our findings indicate that CAR would be a novel and prominent marker for separating PSC- and embryo-derived Flk1⁺ mesodermal cells with distinct differentiation potentials. |
| 巻・号 | 4(5) |
| ページ | 424-36 |
| 公開日 | 2015-5-1 |
| DOI | 10.5966/sctm.2014-0173 |
| PII | sctm.2014-0173 |
| PMID | 25762001 |
| PMC | PMC4414217 |
| MeSH | Animals Cell Differentiation / genetics Cell Lineage Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Endothelial Cells / cytology Gene Expression Regulation, Viral / genetics Hematopoietic Stem Cells / metabolism* Humans Mesoderm / cytology Mesoderm / metabolism Mice Receptors, Virus / biosynthesis* Receptors, Virus / genetics |
| IF | 6.429 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BRC6(AES0010) OP9(RCB1124) |