RRC ID |
6159
|
著者 |
Kusube M, Nishino T, Nishikawa Y, Goto M, Matsuki H, Iwahashi H.
|
タイトル |
Recovery of tobacco BY-2 cells after high hydrostatic pressure treatment.
|
ジャーナル |
Ann N Y Acad Sci
|
Abstract |
The recovery of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells in Linsmaire and Skoog medium after treatment at high hydrostatic pressure was investigated using an Evans Blue staining method to discriminate live from dead cells. The survival of BY-2 cells just after the high-pressure treatment at 5 degrees C and 25 degrees C decreased abruptly at pressures higher than 50 MPa and 100 MPa, respectively. Furthermore, almost all of the BY-2 cells treated at 5 degrees C and 25 degrees C recovered pressures below 25 MPa and 75 MPa, respectively. However, no BY-2 cells recovered at pressures above 100 MPa at either temperature.
|
巻・号 |
1189
|
ページ |
139-42
|
公開日 |
2010-2-1
|
DOI |
10.1111/j.1749-6632.2009.05185.x
|
PII |
NYAS5185
|
PMID |
20233380
|
MeSH |
Biotechnology
Cell Line
Cell Proliferation
Cell Survival
Hydrostatic Pressure*
Preservation, Biological / methods*
Temperature
Tobacco / cytology*
|
IF |
4.728
|
引用数 |
0
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00001 |