| RRC ID |
61590
|
| 著者 |
Aoyama E, Watari I, Podyma-Inoue KA, Yanagishita M, Ono T.
|
| タイトル |
Expression of glucagon-like peptide-1 receptor and glucose‑dependent insulinotropic polypeptide receptor is regulated by the glucose concentration in mouse osteoblastic MC3T3-E1 cells.
|
| ジャーナル |
Int J Mol Med
|
| Abstract |
Glucose-dependent insulinotropic polypeptide receptor (GIPR) and glucagon-like peptide-1 receptor (GLP‑1R) are incretin receptors that play important roles in regulating insulin secretion from pancreatic β cells. Incretin receptors are also thought to play a potential role in bone metabolism. Osteoblasts in animals and humans express GIPR; however, the presence of GLP-1R in these cells has not been reported to date. Thus, the aim of this study was to determine whether GLP-1R and GIPR are expressed in osteoblastic cells, and whether their expression levels are regulated by the extracellular glucose concentration. Mouse osteoblastic MC3T3-E1 cells were cultured in medium containing normal (5.6 mM) or high (10, 20 or 30 mM) glucose concentrations, with or without bone morphogenetic protein-2 (BMP-2). RT-PCR, western blot analysis and immunofluorescence were carried out to determine GIPR and GLP-1R mRNA and protein expression levels. Cell proliferation was also assessed. The GLP-1R and GIPR mRNA expression levels were higher in the MC3T3-E1 cells cultured in medium containing high glucose concentrations with BMP-2 compared with the cells cultured in medium containing normal glucose concentrations with or without BMP-2. GLP-1R protein expression increased following culture in high-glucose medium with BMP-2 compared with culture under normal glucose conditions. However, the cellular localization of GLP-1R was not affected by either glucose or BMP-2. In conclusion, our data demonstrate that the expression of GLP-1R and GIPR is regulated by glucose concentrations in MC3T3-E1 cells undergoing differentiation induced by BMP-2. Our results reveal the potential role of incretins in bone metabolism.
|
| 巻・号 |
34(2)
|
| ページ |
475-82
|
| 公開日 |
2014-8-1
|
| DOI |
10.3892/ijmm.2014.1787
|
| PMID |
24866833
|
| MeSH |
Animals
Bone Morphogenetic Protein 2 / metabolism
Cell Differentiation / genetics
Cell Line
Gastric Inhibitory Polypeptide
Gene Expression Regulation
Glucagon-Like Peptide 1 / metabolism
Glucagon-Like Peptide-1 Receptor
Glucose / metabolism*
Humans
Insulin / metabolism*
Mice
Osteoblasts / metabolism
Receptors, Gastrointestinal Hormone / biosynthesis*
Receptors, Gastrointestinal Hormone / metabolism
Receptors, Glucagon / biosynthesis*
Receptors, Glucagon / metabolism
|
| IF |
3.098
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
