| RRC ID |
61599
|
| Author |
Hirono M, Saijo-Kurita K, Kurashima J, Watanabe Y, Ohno T.
|
| Title |
Expression of Tetrahymena actin in mammalian cells.
|
| Journal |
Cell Biol Int Rep
|
| Abstract |
We previously revealed that Tetrahymena actin can copolymerize with rabbit skeletal muscle actin whereas it has a very divergent primary structure and some unusual properties. To investigate the effects of coexistence of this unusual Tetrahymena actin in mammalian cells, we here transfected Tetrahymena actin gene on an expression vector into COS-1 cells. From the results of immunofluorescence microscopy, it was found that Tetrahymena actin expressed in COS-1 cells copolymerized with intrinsic actin, and it was conspicuously localized to the tips of microfilament core bundles in microspikes. On the other hand, increase in cell number tended to cease temporarily about 24 hr after transfection with Tetrahymena actin gene, implying the inhibition of cytokinesis by Tetrahymena actin coexistence.
|
| Volume |
16(7)
|
| Pages |
645-51
|
| Published |
1992-7-1
|
| DOI |
10.1016/s0309-1651(06)80006-8
|
| PMID |
1516142
|
| MeSH |
Actin Cytoskeleton / metabolism
Actins / genetics*
Actins / metabolism
Animals
Cell Division
Cell Line
Fluorescent Antibody Technique
Gene Expression
Plasmids
Polymers / metabolism
Tetrahymena / genetics*
Transfection
|
| Resource |
| Human and Animal Cells |
COS-1(RCB0143) |
