RRC ID 61599
著者 Hirono M, Saijo-Kurita K, Kurashima J, Watanabe Y, Ohno T.
タイトル Expression of Tetrahymena actin in mammalian cells.
ジャーナル Cell Biol Int Rep
Abstract We previously revealed that Tetrahymena actin can copolymerize with rabbit skeletal muscle actin whereas it has a very divergent primary structure and some unusual properties. To investigate the effects of coexistence of this unusual Tetrahymena actin in mammalian cells, we here transfected Tetrahymena actin gene on an expression vector into COS-1 cells. From the results of immunofluorescence microscopy, it was found that Tetrahymena actin expressed in COS-1 cells copolymerized with intrinsic actin, and it was conspicuously localized to the tips of microfilament core bundles in microspikes. On the other hand, increase in cell number tended to cease temporarily about 24 hr after transfection with Tetrahymena actin gene, implying the inhibition of cytokinesis by Tetrahymena actin coexistence.
巻・号 16(7)
ページ 645-51
公開日 1992-7-1
DOI 10.1016/s0309-1651(06)80006-8
PMID 1516142
MeSH Actin Cytoskeleton / metabolism Actins / genetics* Actins / metabolism Animals Cell Division Cell Line Fluorescent Antibody Technique Gene Expression Plasmids Polymers / metabolism Tetrahymena / genetics* Transfection
リソース情報
ヒト・動物細胞 COS-1(RCB0143)