RRC ID 6160
Author Shibata N, Kagiyama M, Nakagawa M, Hirano Y, Hakoshima T.
Title Crystallization of the plant hormone receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid.
Journal Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
Abstract Abscisic acid (ABA) is a plant hormone that plays key regulatory roles in physiological pathways for the adaptation of vegetative tissues to abiotic stresses such as water stress in addition to events pertaining to plant growth and development. The Arabidopsis ABA receptor proteins PYR/PYLs/RCARs form a START family that contains 14 members which are classified into three subfamilies (I-III). Here, purification, crystallization and X-ray data collection are reported for a member of each of the subfamilies, PYL9/RCAR1 from subfamily I, PYL5/RCAR8 from subfamily II and PYR1/RCAR11 from subfamily III, in the presence of (+)-abscisic acid. The three proteins crystallize in space groups P3(1)21/P3(2)21, P2 and P1, respectively. X-ray intensity data were collected to 1.9-2.6 A resolution.
Volume 66(Pt 4)
Pages 456-9
Published 2010-4-1
DOI 10.1107/S1744309110007037
PII S1744309110007037
PMID 20383021
PMC PMC2852343
MeSH Abscisic Acid / chemistry* Arabidopsis / chemistry* Arabidopsis Proteins / chemistry* Carrier Proteins / chemistry* Crystallization Crystallography, X-Ray Membrane Transport Proteins / chemistry*
IF 1.199
Times Cited 6
WOS Category CRYSTALLOGRAPHY BIOCHEMICAL RESEARCH METHODS BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda18331 pda03718 pda01246