RRC ID |
6160
|
著者 |
Shibata N, Kagiyama M, Nakagawa M, Hirano Y, Hakoshima T.
|
タイトル |
Crystallization of the plant hormone receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid.
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ジャーナル |
Acta Crystallogr Sect F Struct Biol Cryst Commun
|
Abstract |
Abscisic acid (ABA) is a plant hormone that plays key regulatory roles in physiological pathways for the adaptation of vegetative tissues to abiotic stresses such as water stress in addition to events pertaining to plant growth and development. The Arabidopsis ABA receptor proteins PYR/PYLs/RCARs form a START family that contains 14 members which are classified into three subfamilies (I-III). Here, purification, crystallization and X-ray data collection are reported for a member of each of the subfamilies, PYL9/RCAR1 from subfamily I, PYL5/RCAR8 from subfamily II and PYR1/RCAR11 from subfamily III, in the presence of (+)-abscisic acid. The three proteins crystallize in space groups P3(1)21/P3(2)21, P2 and P1, respectively. X-ray intensity data were collected to 1.9-2.6 A resolution.
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巻・号 |
66(Pt 4)
|
ページ |
456-9
|
公開日 |
2010-4-1
|
DOI |
10.1107/S1744309110007037
|
PII |
S1744309110007037
|
PMID |
20383021
|
PMC |
PMC2852343
|
MeSH |
Abscisic Acid / chemistry*
Arabidopsis / chemistry*
Arabidopsis Proteins / chemistry*
Carrier Proteins / chemistry*
Crystallization
Crystallography, X-Ray
Intracellular Signaling Peptides and Proteins
Membrane Transport Proteins / chemistry*
|
IF |
0.968
|
引用数 |
6
|
WOS 分野
|
CRYSTALLOGRAPHY
BIOCHEMICAL RESEARCH METHODS
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda18331
pda03718
pda01246 |