論文 - 詳細
| RRC ID | 61614 |
|---|---|
| 著者 | Sasaki J, Asoh TA, Matsumoto T, Egusa H, Sohmura T, Alsberg E, Akashi M, Yatani H. |
| タイトル | Fabrication of three-dimensional cell constructs using temperature-responsive hydrogel. |
| ジャーナル | Tissue Eng Part A |
| Abstract |
A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study. |
| 巻・号 | 16(8) |
| ページ | 2497-504 |
| 公開日 | 2010-8-1 |
| DOI | 10.1089/ten.TEA.2009.0523 |
| PMID | 20218862 |
| MeSH | Acrylic Resins / chemistry* Animals Biocompatible Materials / chemistry* Cell Culture Techniques / methods* Cell Line Hydrogels / chemistry* Materials Testing Mice Osteoblasts / cytology* Osteoblasts / physiology* Surface Properties Temperature Tissue Engineering / methods* |
| IF | 3.508 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |