| RRC ID |
61614
|
| 著者 |
Sasaki J, Asoh TA, Matsumoto T, Egusa H, Sohmura T, Alsberg E, Akashi M, Yatani H.
|
| タイトル |
Fabrication of three-dimensional cell constructs using temperature-responsive hydrogel.
|
| ジャーナル |
Tissue Eng Part A
|
| Abstract |
A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.
|
| 巻・号 |
16(8)
|
| ページ |
2497-504
|
| 公開日 |
2010-8-1
|
| DOI |
10.1089/ten.TEA.2009.0523
|
| PMID |
20218862
|
| MeSH |
Acrylic Resins / chemistry*
Animals
Biocompatible Materials / chemistry*
Cell Culture Techniques / methods*
Cell Line
Hydrogels / chemistry*
Materials Testing
Mice
Osteoblasts / cytology*
Osteoblasts / physiology*
Surface Properties
Temperature
Tissue Engineering / methods*
|
| IF |
3.508
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
