Reference - Detail
| RRC ID | 61638 |
|---|---|
| Author | Anung Wahyudi, Reiko Motohashi |
| Title | The methods of particle bombardment and observation of sGFP signals |
| Journal | AIP Conference Proceedings |
| Abstract |
In these study, we introduced 35Sp::sGFP-SlTIL1, 35Sp::sGFP-SlTIL2, 35Sp::sGFP-SlCHL, 35Sp::SlTIL1- sGFP, 35Sp::SlTIL2-sGFP, and 35Sp::SlCHL-sGFP constructs into onion (Allium cepa) epidermal cells, Nicotiana tabacum (SR1) and Nicotiana benthamiana leaf cells by a particle bombardment PDS-1000 System (Bio-Rad, Hercules, CA, USA). Transient expression of the sGFP-SlTIL1, sGFP-SlTIL2, sGFP-SlCHL, SlTIL1-sGFP, SlTIL2-sGFP, and SlCHL-sGFP fusion proteins were observed by a confocal laser scanning microscopy (Leica SP, Solms, Germany and LSM 700, Carl Zeiss).The result showed that the fusion proteins were accumulated in the plasma membrane and around membranes of the onion (A. cepa) epidermal cells. In case of N. tabacumleaf cells, sometimes detected the fluorescence around plastids and nuclear and in reticulate structures. In the control cells expressing sGFP (pUGW5-sGFP and pUGW6-sGFP), the fluorescence was found in the cytosol and the nucleus. The fusion proteins of SlCHL-sGFP were localized in the chloroplasts of N. tabacum and N. benthamiana leaf cells. |
| Volume | 2296(1) |
| Published | 2020-11-16 |
| DOI | 10.1063/5.0030466 |
| Resource | |
| Tomato | SlTIL1(LEFL2018O11) ,SlTIL2(LEFL1002CA07),SlCHL(LEFL2044D22) |