論文 - 詳細
| RRC ID | 61646 |
|---|---|
| 著者 | Kutsuna T, Inoue H, Takeda H, Takahashi T, Yamamoto H, Miura H, Higashiyama S. |
| タイトル | Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis. |
| ジャーナル | Int J Mol Med |
| Abstract |
Transforming growth factor (TGF)-β and bone morphogenetic protein (BMP) induce a cartilage-specific extracellular matrix (ECM) gene, aggrecan, in a chondrogenic cell line, ATDC5. The results of our recent study show that TGF-β1, but not BMP-4, strongly induces an ECM gene, fibronectin, during chondrogenesis. However, the role of fibronectin in chondrogenesis is unclear. In the current study, our results showed that TGF-β1, but not BMP-4, led to versican-dominant proteoglycan production during chondrogenesis of ATDC5 cells. siRNA-mediated reduction of fibronectin and interference in the liaison between fibronectin and integrins by the Arg-Gly-Asp-Ser (RGDS) peptide increased aggrecan expression, and decreased versican expression by TGF-β1 stimulation. These data suggest that fibronectin is a critical mediator for TGF-β-specific production balance of 2 major proteoglycans, aggrecan and versican, during chondrogenesis. |
| 巻・号 | 28(5) |
| ページ | 829-34 |
| 公開日 | 2011-11-1 |
| DOI | 10.3892/ijmm.2011.766 |
| PMID | 21822533 |
| MeSH | Animals Blotting, Western Cell Line Chondrogenesis / drug effects* Chondrogenesis / genetics Fibronectins / genetics Fibronectins / metabolism* Models, Biological Proteoglycans / biosynthesis* Reverse Transcriptase Polymerase Chain Reaction Transforming Growth Factor beta1 / pharmacology* |
| IF | 3.098 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |