論文 - 詳細
| RRC ID | 6165 |
|---|---|
| 著者 | Mikkelsen MD, Olsen CE, Halkier BA. |
| タイトル | Production of the cancer-preventive glucoraphanin in tobacco. |
| ジャーナル | Mol Plant |
| Abstract |
Epidemiological studies have demonstrated reduced risk of developing cancer upon consumption of diets rich in cruciferous vegetables. This chemoprevention has been largely attributed to the presence of the natural products glucosinolates, particularly the methionine-derived glucoraphanin from broccoli. Improved nutrition by functional foods or health-promoting dietary supplements is an attractive means for prevention of lifestyle-based diseases. Towards this goal, we have engineered the glucoraphanin pathway into tobacco. First, we engineered elongation of the side chain of methionine to produce the key intermediate dihomo-methionine. This process is catalyzed through two cycles in a chain-elongation pathway that takes place partly in the cytosol and partly in the chloroplast. Second, by coupling the five enzymes of the chain-elongation pathway to eight enzymes of the glucosinolate pathway, we show production of glucoraphanin together with other glucosinolates derived from chain-elongated isoleucine and/or leucine. The conversion of methionine to glucoraphanin is obtained via 14 intermediates. Demonstrating the production of the high-value glucoraphanin in a heterologous host has great potential in the food and medicinal industry as a means to generate a stable, rich source of glucoraphanin for the benefit of human health. |
| 巻・号 | 3(4) |
| ページ | 751-9 |
| 公開日 | 2010-7-1 |
| DOI | 10.1093/mp/ssq020 |
| PII | S1674-2052(14)60526-8 |
| PMID | 20457641 |
| MeSH | Glucosinolates / biosynthesis* Glucosinolates / therapeutic use* Imidoesters / therapeutic use* Neoplasms / prevention & control* Oximes Sulfoxides Tobacco / chemistry* |
| IF | 12.084 |
| 引用数 | 55 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda08837 |