| RRC ID |
6166
|
| Author |
Nakajima K, Uchiumi T, Okamoto T.
|
| Title |
Positional relationship between the gamete fusion site and the first division plane in the rice zygote.
|
| Journal |
J Exp Bot
|
| Abstract |
In angiosperms, a zygote generally divides into a two-celled proembryo consisting of an apical and a basal cell that possess different cell fates. This first division of the zygote is a putative step in the formation of the apical-basal axis of the proembryo. The gamete fusion activates the egg, and the gamete fusion site on the zygote has been reported to provide a possible cue for subsequent zygotic development and/or embryonic patterning in animals and plants. In this study, the gamete fusion site on the rice zygote was labelled by in vitro fertilization of a rice egg cell with a fluorescence-stained sperm cell. The positional relationship between the gamete fusion site and the division plane formed by zygotic cleavage was monitored using a fixed culture of the fusion site-labelled zygote until the two-celled proembryo stage. The results indicate that gamete fusion sites exist on two-celled proembryos with no relation to the position of the first division plane, and that the gamete fusion site on the rice zygote does not function as a determinant for positioning the zygote division plane.
|
| Volume |
61(11)
|
| Pages |
3101-5
|
| Published |
2010-6-1
|
| DOI |
10.1093/jxb/erq131
|
| PII |
erq131
|
| PMID |
20462944
|
| PMC |
PMC2892148
|
| MeSH |
Cell Division
Cell Fusion
Germ Cells, Plant / cytology*
Oryza / cytology*
Zygote / cytology*
|
| IF |
5.908
|
| Times Cited |
14
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00031 |
