論文 - 詳細
| RRC ID | 61673 |
|---|---|
| 著者 | Awong G, Zúñiga-Pflücker JC. |
| タイトル | Generation, isolation, and engraftment of in vitro-derived human T cell progenitors. |
| ジャーナル | Methods Mol Biol |
| Abstract |
T cells typically differentiate via a series of coordinated steps within the highly specialized microenvironment of the thymus. Traditionally, human T-lymphopoiesis in vitro has been studied using the hybrid human/mouse fetal thymic organ culture system. Pioneering work by McCune et al. devised a method to examine human T cell development in vivo in relation to HIV-1 using the SCID/hu (thy/liv) model. This was followed by models that better reflected the ability of human hematopoietic cells to home and differentiate within the mouse host without human fetal tissues; however, human T cell development in these animals was poor. In this chapter, we outline a procedure to generate human progenitor T (proT) cells in vitro from umbilical cord blood-derived hematopoietic stem cells using the OP9-DL1 cell system; in addition, we describe the method used to examine the engraftment of in vitro-derived proT cells into immunodeficient mouse strains. |
| 巻・号 | 946 |
| ページ | 103-13 |
| 公開日 | 2013-1-1 |
| DOI | 10.1007/978-1-62703-128-8_7 |
| PMID | 23179828 |
| MeSH | Animals Cell Line Cell Separation / methods* Coculture Techniques Flow Cytometry Humans Infant, Newborn Liver / cytology Lymphoid Progenitor Cells / cytology* Lymphoid Progenitor Cells / transplantation* Mice Stem Cell Transplantation / methods* Stromal Cells / cytology T-Lymphocytes / cytology* Umbilical Cord / cytology |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |