| RRC ID |
61675
|
| 著者 |
Itoh H, Kataoka H, Yamauchi M, Naganuma S, Akiyama Y, Nuki Y, Shimomura T, Miyazawa K, Kitamura N, Koono M.
|
| タイトル |
Identification of hepatocyte growth factor activator inhibitor type 2 (HAI-2)-related small peptide (H2RSP): its nuclear localization and generation of chimeric mRNA transcribed from both HAI-2 and H2RSP genes.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
A novel small gene, designated hepatocyte growth factor activator inhibitor type 2 (HAI-2)-related small peptide (H2RSP) was cloned and characterized in the process of the search for splicing variant forms of HAI-2 by 3'-rapid amplification of cDNA ends (RACE). The gene consisted of 4 exons spanning approximately 1 kb and was located in 11 kb downstream of HAI-2 gene (19q.13.11). The novel transcript identified by 3'-RACE was thought to be chimerically transcribed from both HAI-2 (exons 1-7) and H2RSP (exons 2-4) genes. Wild-type H2RSP mRNA (0.5 kb) was detected abundantly in various tissues including the gastrointestinal tract, whereas chimeric mRNA (1.5 kb) was found mainly in the kidney, prostate, and placenta by Northern blot analysis. The predicted amino acid sequence of H2RSP contained two unique domains, namely the serine-rich region (exon 3) and the lysine-rich region (exon 4). Transfection of deleted series of H2RSP cDNAs fused to enhanced green fluorescent protein (EGFP) into HeLa cells revealed that H2RSP has nuclear localization signal in the lysine-rich region. Immunohistochemical study using anti-H2RSP polyclonal antibody indeed revealed the nuclear localization of this peptide in vivo. These results suggest that H2RSP and H2RSP/HAI-2 chimeric peptides might function as a transcriptional regulatory peptide at the nucleus.
|
| 巻・号 |
288(2)
|
| ページ |
390-9
|
| 公開日 |
2001-10-26
|
| DOI |
10.1006/bbrc.2001.5767
|
| PII |
S0006-291X(01)95767-5
|
| PMID |
11606055
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
CHO Cells
Cell Nucleus / metabolism*
Cricetinae
DNA, Complementary / analysis
DNA, Complementary / isolation & purification
Green Fluorescent Proteins
HeLa Cells
Hepatocyte Growth Factor / metabolism
Humans
Immunohistochemistry
Luminescent Proteins / metabolism
Membrane Glycoproteins / analysis
Membrane Glycoproteins / genetics
Molecular Sequence Data
Nuclear Proteins / analysis
Nuclear Proteins / genetics*
RNA, Messenger / metabolism
Recombinant Fusion Proteins / metabolism
Serine Endopeptidases / metabolism*
Subcellular Fractions
Tissue Distribution
Transcription Factors / analysis
Transcription Factors / genetics*
Trypsin Inhibitor, Kunitz Soybean*
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001)
CHO-K1(RCB0285) |
