論文 - 詳細
| RRC ID | 61682 |
|---|---|
| 著者 | Sunarso, Toita R, Tsuru K, Ishikawa K. |
| タイトル | Immobilization of calcium and phosphate ions improves the osteoconductivity of titanium implants. |
| ジャーナル | Mater Sci Eng C Mater Biol Appl |
| Abstract |
In this work, to elevate weak osteoconductivity of titanium (Ti) implant, we prepared a Ti implant having both calcium and phosphate ions on its surface. To modify calcium and phosphate ions onto Ti, phosphate ions were first immobilized by treating the Ti with a NaH2PO4 solution, followed by CaCl2 treatment to immobilize calcium ions, which created the calcium and phosphate ions-modified Ti (Ca-P-Ti). X-ray photoelectron spectroscopy and thin-layer X-ray diffraction measurement confirmed that both phosphate and calcium ions were co-immobilized onto the Ti surface on the molecular level. Three-hour after seeding MC3T3-E1 murine pre-osteoblast cells on substrates, cell number on Ca-P-Ti was much larger than that of Ti and phosphate-modified Ti (P-Ti), but was similar to that of calcium-modified Ti (Ca-Ti). Also, MC3T3-E1 cells on Ca-P-Ti expressed larger amount of vinculin, a focal adhesion protein, than those on other substrates, probably resulting in larger cell size as well as greater cell proliferation on Ca-P-Ti than those on other substrates. Alkaline phosphatase activity of cells on Ca-P-Ti was greater than those on Ti and P-Ti, but was almost comparable to that of Ca-Ti. Moreover, the largest amount of bone-like nodule formation was observed on Ca-P-Ti. These results provide evidence that calcium and phosphate ions-co-immobilization onto Ti increased the osteoconductivity of Ti by stimulating the responses of pre-osteoblast cells. This simple modification would be promising technique for bone tissue implant including dental and orthopedic implants. |
| 巻・号 | 68 |
| ページ | 291-298 |
| 公開日 | 2016-11-1 |
| DOI | 10.1016/j.msec.2016.05.090 |
| PII | S0928-4931(16)30523-9 |
| PMID | 27524023 |
| MeSH | Animals Calcium* / chemistry Calcium* / pharmacokinetics Calcium* / pharmacology Coated Materials, Biocompatible* / chemistry Coated Materials, Biocompatible* / pharmacokinetics Coated Materials, Biocompatible* / pharmacology Mice Osteoblasts / metabolism* Osteogenesis / drug effects* Phosphates* / chemistry Phosphates* / pharmacokinetics Phosphates* / pharmacology Titanium* / chemistry Titanium* / pharmacokinetics Titanium* / pharmacology |
| IF | 5.88 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |