RRC ID 61682
著者 Sunarso, Toita R, Tsuru K, Ishikawa K.
タイトル Immobilization of calcium and phosphate ions improves the osteoconductivity of titanium implants.
ジャーナル Mater Sci Eng C Mater Biol Appl
Abstract In this work, to elevate weak osteoconductivity of titanium (Ti) implant, we prepared a Ti implant having both calcium and phosphate ions on its surface. To modify calcium and phosphate ions onto Ti, phosphate ions were first immobilized by treating the Ti with a NaH2PO4 solution, followed by CaCl2 treatment to immobilize calcium ions, which created the calcium and phosphate ions-modified Ti (Ca-P-Ti). X-ray photoelectron spectroscopy and thin-layer X-ray diffraction measurement confirmed that both phosphate and calcium ions were co-immobilized onto the Ti surface on the molecular level. Three-hour after seeding MC3T3-E1 murine pre-osteoblast cells on substrates, cell number on Ca-P-Ti was much larger than that of Ti and phosphate-modified Ti (P-Ti), but was similar to that of calcium-modified Ti (Ca-Ti). Also, MC3T3-E1 cells on Ca-P-Ti expressed larger amount of vinculin, a focal adhesion protein, than those on other substrates, probably resulting in larger cell size as well as greater cell proliferation on Ca-P-Ti than those on other substrates. Alkaline phosphatase activity of cells on Ca-P-Ti was greater than those on Ti and P-Ti, but was almost comparable to that of Ca-Ti. Moreover, the largest amount of bone-like nodule formation was observed on Ca-P-Ti. These results provide evidence that calcium and phosphate ions-co-immobilization onto Ti increased the osteoconductivity of Ti by stimulating the responses of pre-osteoblast cells. This simple modification would be promising technique for bone tissue implant including dental and orthopedic implants.
巻・号 68
ページ 291-298
公開日 2016-11-1
DOI 10.1016/j.msec.2016.05.090
PII S0928-4931(16)30523-9
PMID 27524023
MeSH Animals Calcium* / chemistry Calcium* / pharmacokinetics Calcium* / pharmacology Coated Materials, Biocompatible* / chemistry Coated Materials, Biocompatible* / pharmacokinetics Coated Materials, Biocompatible* / pharmacology Mice Osteoblasts / metabolism* Osteogenesis / drug effects* Phosphates* / chemistry Phosphates* / pharmacokinetics Phosphates* / pharmacology Titanium* / chemistry Titanium* / pharmacokinetics Titanium* / pharmacology
IF 5.88
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)