RRC ID 61686
Author Autsavapromporn N, Liu C, Konishi T.
Title Impact of Co-Culturing with Fractionated Carbon-Ion-Irradiated Cancer Cells on Bystander Normal Cells and Their Progeny.
Journal Radiat Res
Abstract The purpose of this study was to compare the biological effects of fractionated doses versus a single dose of high-LET carbon ions in bystander normal cells, and determine the effect on their progeny using the layered tissue co-culture system. Briefly, confluent human glioblastoma (T98G) cells received a single dose of 6 Gy or three daily doses of 2 Gy carbon ions, which were then seeded on top of an insert with bystander normal skin fibroblasts (NB1RGB) growing underneath. Cells were co-cultured for 6 h or allowed to grow for 20 population doublings, then harvested and assayed for different end points. A single dose of carbon ions resulted in less damage in bystander normal NB1RGB cells than the fractionated doses. In contrast, the progeny of bystander NB1RGB cells co-cultured with T98G cells exposed to fractionated doses showed less damage than progeny from bystander cells co-cultured with single dose glioblastoma cells. Furthermore, inhibition of gap junction communication demonstrated its involvement in the stressful effects in bystander cells and their progeny. These results indicate that dose fractionation reduced the late effect of carbon-ion exposure in the progeny of bystander cells compared to the effect in the initial bystander cells.
Volume 188(3)
Pages 335-341
Published 2017-9-1
DOI 10.1667/RR14773.1
PMID 28686544
MeSH Bystander Effect / physiology* Bystander Effect / radiation effects* Cell Line Cell Survival / physiology Cell Survival / radiation effects* Dose Fractionation, Radiation* Dose-Response Relationship, Radiation Fibroblasts / cytology Fibroblasts / physiology Fibroblasts / radiation effects* Heavy Ion Radiotherapy / methods Humans Neoplasms, Experimental / pathology Neoplasms, Experimental / physiopathology Neoplasms, Experimental / radiotherapy* Treatment Outcome
IF 2.779
Human and Animal Cells NB1RGB(RCB0222)