論文 - 詳細
| RRC ID | 61714 |
|---|---|
| 著者 | Yamagishi T, Yamaguchi H, Suzuki S, Yoshikawa M, Jameson I, Lorenz M, Nobles DR, Campbell C, Seki M, Kawachi M, Yamamoto H. |
| タイトル | Comparative genome analysis of test algal strain NIVA-CHL1 (Raphidocelis subcapitata) maintained in microalgal culture collections worldwide. |
| ジャーナル | PLoS One |
| Abstract |
Raphidocelis subcapitata is one of the most frequently used species for algal growth inhibition tests. Accordingly, many microalgal culture collections worldwide maintain R. subcapitata for distribution to users. All R. subcapitata strains maintained in these collections are derived from the same cultured strain, NIVA-CHL1. However, considering that 61 years have passed since this strain was isolated, we suspected that NIVA-CHL1 in culture collections might have acquired various mutations. In this study, we compared the genome sequences among NIVA-CHL1 from 8 microalgal culture collections and one laboratory in Japan to evaluate the presence of mutations. We found single-nucleotide polymorphisms or indels at 19,576 to 28,212 sites per strain in comparison with the genome sequence of R. subcapitata NIES-35, maintained at the National Institute for Environmental Studies, Tsukuba, Japan. These mutations were detected not only in non-coding but also in coding regions; some of the latter mutations may affect protein function. In growth inhibition test with 3,5-dichlorophenol, EC50 values varied 2.6-fold among the 9 strains. In the ATCC 22662-2 and CCAP 278/4 strains, we also detected a mutation in the gene encoding small-conductance mechanosensitive ion channel, which may lead to protein truncation and loss of function. Growth inhibition test with sodium chloride suggested that osmotic regulation has changed in ATCC 22662-2 and CCAP 278/4 in comparison with NIES-35. |
| 巻・号 | 15(11) |
| ページ | e0241889 |
| 公開日 | 2020-1-1 |
| DOI | 10.1371/journal.pone.0241889 |
| PII | PONE-D-20-26198 |
| PMID | 33166324 |
| PMC | PMC7652255 |
| MeSH | Algal Proteins / drug effects Algal Proteins / genetics* Chlorophyceae / drug effects Chlorophyceae / genetics* Chlorophyceae / growth & development* Culture Media / chemistry Gene Expression Regulation / drug effects Japan Polymorphism, Single Nucleotide* Sodium Chloride / pharmacology* Whole Genome Sequencing / methods* |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 44 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-35 |