| RRC ID |
61722
|
| 著者 |
Imura K, Ueda Y, Hayashi T, Itoh T, Shimizu K, Tamai H, Yano Y, Naito K, Kohara J, Nakane K, Matsuura Y, Takeda A, Takeda T, Kawai K, Yamagishi H.
|
| タイトル |
Induction of cytotoxic T lymphocytes against human cancer cell lines using dendritic cell-tumor cell hybrids generated by a newly developed electrofusion technique.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
Recently, dendritic cells (DCs) and DC-tumor cell hybrids (DC-tumor hybrids) have been used for cancer vaccine therapy in a clinical trial. DC-tumor hybrids combine the potent antigen-presenting capacity of DCs with the ability to present all tumor antigens expressed on tumor cells to T cells. We used DC-tumor hybrids as stimulator cells to induce tumor-specific cytotoxic T lymphocytes (CTLs) in vitro. DC-tumor hybrids were generated from human monocyte-derived DCs and human cancer-cell lines (GT3TKB, lung cancer; GCIY, gastric cancer) by our newly developed electrofusion technique, established and refined with the use of mouse cells. To evaluate the capacity of DC-tumor hybrids generated by our method to induce tumor antigen-specific CTLs, we performed a cytotoxic assay and an interferon-gamma release assay using CD8-dominant effector lymphocytes induced by them. DC-tumor hybrids more effectively induced tumor-specific primary T-cell response than did stimulation with DCs co-cultured with irradiated tumor cells overnight, irradiated tumor cells alone, or a mixture of DCs and irradiated tumor cells. DC-tumor hybrids were generated at a high fusion rate by our electrofusion technique. When CTLs were induced by DC-tumor hybrids in vitro, the high fusion rate did not contribute to the induction of CTLs with increased tumor-specific cytotoxicity. The addition of interleukin-12 to the culture medium did not augment the cytotoxicity of CTLs. Overall, our results suggest that DC-tumor hybrids effectively induce human tumor-specific CTLs and may thus be applicable for clinical trials of adoptive immunotherapy.
|
| 巻・号 |
29(3)
|
| ページ |
531-9
|
| 公開日 |
2006-9-1
|
| PMID |
16865268
|
| MeSH |
Adenocarcinoma / immunology
Adenocarcinoma / pathology
Adult
Antigens, Neoplasm / immunology
Antigens, Neoplasm / metabolism
Antigens, Neoplasm / therapeutic use*
Antineoplastic Agents / pharmacology
CD8-Positive T-Lymphocytes / immunology
Carcinoma, Squamous Cell / immunology
Carcinoma, Squamous Cell / pathology
Cell Fusion
Dendritic Cells / immunology*
Humans
Hybrid Cells / immunology
Interferon-gamma / metabolism
Interleukin-12 / pharmacology
Lung Neoplasms / immunology*
Lung Neoplasms / pathology
Male
Stomach Neoplasms / immunology*
Stomach Neoplasms / pathology
T-Lymphocytes, Cytotoxic / immunology*
Tumor Cells, Cultured
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
GCIY(RCB0555) |
