論文 - 詳細
| RRC ID | 61724 |
|---|---|
| 著者 | Chan MW, Wong CY, Cheng AS, Chan VY, Chan KK, To KF, Chan FK, Sung JJ, Leung WK. |
| タイトル | Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells. |
| ジャーナル | Oncol Rep |
| Abstract |
Although selective cyclooxygenase-2 (COX-2) inhibitors suppress cell proliferation in gastric cancer, it remains debatable whether their effect is mediated through COX-2 dependent or independent pathways. We investigated the effects of the targeted inhibition of COX-2 expression by small interfering RNA (siRNA) in human gastric cancer cells and compared it to the effects of treatment with a specific COX-2 inhibitor. COX-2 mRNA and proteins were significantly reduced by up to 80% on day 2 after COX-2 siRNA transfection to the gastric cancer cell line MKN45. Concentrations of prostaglandins E2 (PGE2) in the condition medium were also reduced to 30% after siRNA transfection. Transfection of COX-2 siRNA exhibited a more potent anti-proliferative effect on MKN45 cells than treatment with high-dose (100 microM) NS398. COX-2 siRNA also significantly reduced tumor growth in nude mice. While COX-2 siRNA transfection alone had no obvious pro-apoptotic effects, unlike low-dose (10 microM) NS398 it enhanced the apoptotic reaction of MKN45 cells to cisplatin therapy. In conclusion, our results demonstrate for the first time that COX-2 siRNA inhibits cell growth and enhances the chemosensitivity of gastric cancer cells. RNA interference may be a promising alternative to specific COX-2 inhibitors in the prevention and treatment of gastric cancer. |
| 巻・号 | 18(6) |
| ページ | 1557-62 |
| 公開日 | 2007-12-1 |
| PMID | 17982644 |
| MeSH | Antineoplastic Agents / therapeutic use Cell Division / drug effects Cell Line, Tumor Cisplatin / therapeutic use* Cyclooxygenase 2 / genetics* Gene Expression Regulation, Enzymologic / drug effects Gene Expression Regulation, Neoplastic / drug effects Humans Plasmids RNA Interference* RNA, Messenger / genetics RNA, Small Interfering / genetics* Stomach Neoplasms / drug therapy Stomach Neoplasms / pathology* |
| IF | 3.417 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) |