論文 - 詳細
| RRC ID | 61736 |
|---|---|
| 著者 | Shindo Y, Yamanaka R, Suzuki K, Hotta K, Oka K. |
| タイトル | Intracellular magnesium level determines cell viability in the MPP(+) model of Parkinson's disease. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Parkinson's disease (PD) is a neurodegenerative disorder resulting from mitochondrial dysfunction in dopaminergic neurons. Mitochondria are believed to be responsible for cellular Mg²⁺ homeostasis. Mg²⁺ is indispensable for maintaining ordinal cellular functions, hence perturbation of the cellular Mg²⁺ homeostasis may be responsible for the disorders of physiological functions and diseases including PD. However, the changes in intracellular Mg²⁺ concentration ([Mg²⁺]i) and the role of Mg²⁺ in PD have still been obscure. In this study, we investigated [Mg²⁺]i and its effect on neurodegeneration in the 1-methyl-4-phenylpyridinium (MPP⁺) model of PD in differentiated PC12 cells. Application of MPP⁺ induced an increase in [Mg²⁺]i immediately via two different pathways: Mg²⁺ release from mitochondria and Mg²⁺ influx across cell membrane, and the increased [Mg²⁺]i sustained for more than 16 h after MPP⁺ application. Suppression of Mg²⁺ influx decreased the viability of the cells exposed to MPP⁺. The cell viability correlated highly with [Mg²⁺]i. In the PC12 cells with suppressed Mg²⁺ influx, ATP concentration decreased and the amount of reactive oxygen species (ROS) increased after an 8h exposure to MPP⁺. Our results indicate that the increase in [Mg²⁺]i inhibited cellular ROS generation and maintained ATP production, which resulted in the protection from MPP⁺ toxicity. |
| 巻・号 | 1853(12) |
| ページ | 3182-91 |
| 公開日 | 2015-12-1 |
| DOI | 10.1016/j.bbamcr.2015.08.013 |
| PII | S0167-4889(15)00282-7 |
| PMID | 26319097 |
| MeSH | 1-Methyl-4-phenylpyridinium / toxicity* Adenosine Triphosphate / metabolism Animals Disease Models, Animal* Dose-Response Relationship, Drug Magnesium / metabolism* PC12 Cells Parkinson Disease / metabolism* Rats |
| IF | 3.411 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |