RRC ID 61767
Author Sawaoka H, Tsuji S, Tsujii M, Gunawan ES, Kawai N, Sasaki Y, Hori M, Kawano S.
Title Involvement of cyclooxygenase-2 in proliferation and morphogenesis induced by transforming growth factor alpha in gastric epithelial cells.
Journal Prostaglandins Leukot Essent Fatty Acids
Abstract Transforming growth factor alpha is one of the most potent growth factors for gastrointestinal epithelium. In this study, we examined the roles of cyclooxygenase-2 on proliferation and morphogenesis of RGM1 rat gastric epithelial cells after stimulation with transforming growth factor alpha in vitro, RGM1 cells increased expression of cyclooxygenase-2 messenger RNA 20-60 min after stimulation with transforming growth factor alpha. Transforming growth factor alpha stimulated [3H]thymidine incorporation and tubulogenesis of RGM1 cells in collagen matrix, both of which were significantly suppressed by treatment with a cyclooxygenase-2 specific inhibitor, NS-398 or cyclooxygenase-2 antisense oligonucleotide. Both of the treatment lowered prostanoid production by enzyme immunoassay. The transforming growth factor alpha-induced expression of cyclooxygenase-2 is followed by cell proliferation and development of tubular morphology of RGM1 gastric epithelial cells. Treatment with cyclooxygenase-2 inhibitor and cyclooxygenase-2 antisense oligonucleotide suppressed these responses induced by transforming growth factor alpha suggesting the involvement of cyclooxygenase-2 in proliferation and morphogenesis in gastric mucosal epithelium.
Volume 61(5)
Pages 315-22
Published 1999-11-1
DOI 10.1054/plef.1999.0106
PII S0952327899901060
PMID 10670694
MeSH 6-Ketoprostaglandin F1 alpha / biosynthesis Animals Base Sequence Cell Differentiation / drug effects* Cell Differentiation / physiology* Cell Division / drug effects* Cell Division / physiology* Cell Line Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cyclooxygenase Inhibitors / pharmacology Epithelial Cells / cytology Epithelial Cells / drug effects Epithelial Cells / enzymology Gastric Mucosa / cytology Gastric Mucosa / drug effects* Gastric Mucosa / enzymology* Gene Expression Isoenzymes / genetics Isoenzymes / pharmacology Isoenzymes / physiology* Kidney Tubules / cytology Kidney Tubules / drug effects Nitrobenzenes / pharmacology Oligodeoxyribonucleotides, Antisense / genetics Oligodeoxyribonucleotides, Antisense / pharmacology Prostaglandin-Endoperoxide Synthases / genetics Prostaglandin-Endoperoxide Synthases / pharmacology Prostaglandin-Endoperoxide Synthases / physiology* RNA, Messenger / genetics RNA, Messenger / metabolism Rats Sulfonamides / pharmacology Transforming Growth Factor alpha / pharmacology*
IF 2.932
Human and Animal Cells RGM1(RCB0876)