| RRC ID |
61767
|
| 著者 |
Sawaoka H, Tsuji S, Tsujii M, Gunawan ES, Kawai N, Sasaki Y, Hori M, Kawano S.
|
| タイトル |
Involvement of cyclooxygenase-2 in proliferation and morphogenesis induced by transforming growth factor alpha in gastric epithelial cells.
|
| ジャーナル |
Prostaglandins Leukot Essent Fatty Acids
|
| Abstract |
Transforming growth factor alpha is one of the most potent growth factors for gastrointestinal epithelium. In this study, we examined the roles of cyclooxygenase-2 on proliferation and morphogenesis of RGM1 rat gastric epithelial cells after stimulation with transforming growth factor alpha in vitro, RGM1 cells increased expression of cyclooxygenase-2 messenger RNA 20-60 min after stimulation with transforming growth factor alpha. Transforming growth factor alpha stimulated [3H]thymidine incorporation and tubulogenesis of RGM1 cells in collagen matrix, both of which were significantly suppressed by treatment with a cyclooxygenase-2 specific inhibitor, NS-398 or cyclooxygenase-2 antisense oligonucleotide. Both of the treatment lowered prostanoid production by enzyme immunoassay. The transforming growth factor alpha-induced expression of cyclooxygenase-2 is followed by cell proliferation and development of tubular morphology of RGM1 gastric epithelial cells. Treatment with cyclooxygenase-2 inhibitor and cyclooxygenase-2 antisense oligonucleotide suppressed these responses induced by transforming growth factor alpha suggesting the involvement of cyclooxygenase-2 in proliferation and morphogenesis in gastric mucosal epithelium.
|
| 巻・号 |
61(5)
|
| ページ |
315-22
|
| 公開日 |
1999-11-1
|
| DOI |
10.1054/plef.1999.0106
|
| PII |
S0952327899901060
|
| PMID |
10670694
|
| MeSH |
6-Ketoprostaglandin F1 alpha / biosynthesis
Animals
Base Sequence
Cell Differentiation / drug effects*
Cell Differentiation / physiology*
Cell Division / drug effects*
Cell Division / physiology*
Cell Line
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors / pharmacology
Epithelial Cells / cytology
Epithelial Cells / drug effects
Epithelial Cells / enzymology
Gastric Mucosa / cytology
Gastric Mucosa / drug effects*
Gastric Mucosa / enzymology*
Gene Expression
Isoenzymes / genetics
Isoenzymes / pharmacology
Isoenzymes / physiology*
Kidney Tubules / cytology
Kidney Tubules / drug effects
Nitrobenzenes / pharmacology
Oligodeoxyribonucleotides, Antisense / genetics
Oligodeoxyribonucleotides, Antisense / pharmacology
Prostaglandin-Endoperoxide Synthases / genetics
Prostaglandin-Endoperoxide Synthases / pharmacology
Prostaglandin-Endoperoxide Synthases / physiology*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Sulfonamides / pharmacology
Transforming Growth Factor alpha / pharmacology*
|
| IF |
2.932
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
