Reference - Detail
| RRC ID | 61770 |
|---|---|
| Author | Furugen A, Ishiguro Y, Kobayashi M, Narumi K, Nishimura A, Hirano T, Iseki K. |
| Title | Involvement of l-type amino acid transporter 1 in the transport of gabapentin into human placental choriocarcinoma cells. |
| Journal | Reprod Toxicol |
| Abstract |
Gabapentin (GBP) is a widely used antiepileptic drug, with potential for use in the treatment of epilepsy in pregnant women. Although studies have examined GBP transport mechanisms across the blood-brain barrier, kidney, and intestine, the mechanism in the placenta has not been fully elucidated. We previously reported that GBP accumulates at high concentrations in human placental choriocarcinoma BeWo cells. The purpose of this study was to examine the transport mechanism of GBP in placental choriocarcinoma cells (BeWo and JEG-3), and to identify the carrier involved. High concentrations of intracellular GBP accumulations were also found in JEG-3 cells. A kinetic analysis showed that a single carrier system was involved in the uptake of GBP. Furthermore, substrates for l-type amino acid transporter (LAT) and siRNAs targeted to LAT1 significantly decreased GBP uptake. Our observations from this study suggest that LAT1 is the main contributor to GBP transport in placental choriocarcinoma cells. |
| Volume | 67 |
| Pages | 48-55 |
| Published | 2017-1-1 |
| DOI | 10.1016/j.reprotox.2016.11.002 |
| PII | S0890-6238(16)30392-6 |
| PMID | 27818298 |
| MeSH | Amines / metabolism Amines / pharmacokinetics* Anticonvulsants / metabolism Anticonvulsants / pharmacokinetics* Biological Transport Blotting, Western Cell Culture Techniques Cell Line, Tumor Cyclohexanecarboxylic Acids / metabolism Cyclohexanecarboxylic Acids / pharmacokinetics* Female Gabapentin Gene Knockdown Techniques Humans Large Neutral Amino Acid-Transporter 1 / genetics Large Neutral Amino Acid-Transporter 1 / metabolism* Placenta / metabolism* Pregnancy Reverse Transcriptase Polymerase Chain Reaction gamma-Aminobutyric Acid / metabolism gamma-Aminobutyric Acid / pharmacokinetics* |
| IF | 3.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | BeWo |