Reference - Detail
| RRC ID | 61777 |
|---|---|
| Author | Tsuda E, Goto M, Mochizuki S, Yano K, Kobayashi F, Morinaga T, Higashio K. |
| Title | Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
A factor which inhibits osteoclast-like cell formation was found in the conditioned medium of human embryonic lung fibroblasts, IMR-90. The factor, termed osteoclastogenesis inhibitory factor, OCIF, was purified to homogeneity. OCIF is a heparin-binding basic glycoprotein and has been isolated as a monomer with an apparent molecular weight (Mr) of 60,000 and a homodimer with a Mr of 120,000. The N-terminus of OCIF is blocked and the determination of internal amino acid sequences revealed that OCIF has no homology to known proteins. OCIF inhibited in a dose-dependent manner osteoclastogenesis elicited through three distinct signaling pathways stimulated by 1 alpha,25-dihydroxy vitamin D3, parathyroid hormone, and interleukin-11, respectively, in a dose range of 1 to 40 ng/ml (IC50 = 4 to 6 ng/ml). OCIF neither inhibits bone resorption by mature osteoclasts nor exerts any other biological activities. These data strongly suggest that OCIF is a novel cytokine which specifically inhibits osteoclastogenesis. |
| Volume | 234(1) |
| Pages | 137-42 |
| Published | 1997-5-8 |
| DOI | 10.1006/bbrc.1997.6603 |
| PII | S0006291X97966031 |
| PMID | 9168977 |
| MeSH | Acid Phosphatase / metabolism Animals Calcitriol / pharmacology Cell Line Chromatography, Affinity Chromatography, High Pressure Liquid Chromatography, Ion Exchange Culture Media, Conditioned Cytokines / chemistry Cytokines / isolation & purification* Cytokines / pharmacology* Electrophoresis, Polyacrylamide Gel Fibroblasts / chemistry* Humans Mice Molecular Weight Osteoclasts / cytology* Osteoclasts / drug effects Parathyroid Hormone / pharmacology Serine Endopeptidases / metabolism |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 62 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ST2(RCB0224) MC3T3-G2/PA6(RCB1127) |