Reference - Detail
| RRC ID | 61784 |
|---|---|
| Author | Shimura Y, Suzuki J, Muraoka M, Kasuya MC, Matsuoka K, Hatanaka K. |
| Title | Large scale biosynthesis of ganglioside analogues by RERF-LC-AI cells cultured in HYPERFlask. |
| Journal | Prep Biochem Biotechnol |
| Abstract |
The efficient production of ganglioside analogues was accomplished using RERF-LC-AI cells cultured in HYPERFlask (High Yield PERformance Flask). Eight kinds of ganglioside analogues (GM3, GM2, sialylparagloboside, GD3, di-sialylated lacto-N-tetraose, and another three kinds of analogues with intricate structures) were synthesized by the saccharide primer method using lung squamous-cell carcinoma line RERF-LC-AI and 12-azidododecyl β-lactoside primer. The yield for each analogue obtained using HYPERFlask was higher than yields obtained from 100-mm dishes. |
| Volume | 42(4) |
| Pages | 378-92 |
| Published | 2012-1-1 |
| DOI | 10.1080/10826068.2011.627971 |
| PMID | 22708814 |
| MeSH | Azides / metabolism Biotechnology / methods* Carbohydrate Sequence Cell Culture Techniques / methods* Cell Line, Tumor Gangliosides / chemistry* Gangliosides / isolation & purification Gangliosides / metabolism* Humans Lactose / analogs & derivatives Lactose / metabolism Lung / cytology* Molecular Sequence Data |
| IF | 1.415 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RERF-LC-AI(RCB0444) |