| RRC ID |
61784
|
| Author |
Shimura Y, Suzuki J, Muraoka M, Kasuya MC, Matsuoka K, Hatanaka K.
|
| Title |
Large scale biosynthesis of ganglioside analogues by RERF-LC-AI cells cultured in HYPERFlask.
|
| Journal |
Prep Biochem Biotechnol
|
| Abstract |
The efficient production of ganglioside analogues was accomplished using RERF-LC-AI cells cultured in HYPERFlask (High Yield PERformance Flask). Eight kinds of ganglioside analogues (GM3, GM2, sialylparagloboside, GD3, di-sialylated lacto-N-tetraose, and another three kinds of analogues with intricate structures) were synthesized by the saccharide primer method using lung squamous-cell carcinoma line RERF-LC-AI and 12-azidododecyl β-lactoside primer. The yield for each analogue obtained using HYPERFlask was higher than yields obtained from 100-mm dishes.
|
| Volume |
42(4)
|
| Pages |
378-92
|
| Published |
2012-1-1
|
| DOI |
10.1080/10826068.2011.627971
|
| PMID |
22708814
|
| MeSH |
Azides / metabolism
Biotechnology / methods*
Carbohydrate Sequence
Cell Culture Techniques / methods*
Cell Line, Tumor
Gangliosides / chemistry*
Gangliosides / isolation & purification
Gangliosides / metabolism*
Humans
Lactose / analogs & derivatives
Lactose / metabolism
Lung / cytology*
Molecular Sequence Data
|
| IF |
1.415
|
| Resource |
| Human and Animal Cells |
RERF-LC-AI(RCB0444) |
