RRC ID 61788
Author Hotta H, Hamamura K, Yamashita K, Shibuya H, Tokuda N, Hashimoto N, Furukawa K, Yamamoto N, Hattori H, Toyokuni S, Ueda M, Furukawa K.
Title Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.
Journal Glycoconj J
Abstract Expression and implication of carbohydrate antigens in squamous cell carcinomas (SCCs) in oral cavity was examined. In the cell lines, type 2H and Lewis y antigens were markedly expressed. In the tissues from SCC patients and benign disorders, type 2H was highly expressed in hyperplasia (96.4 %), displasia (92.9 %) and SCC (100 %). Lewis y was, in turn, expressed mainly in cancer tissues (91.3 %), suggesting that Lewis y is a cancer-associated antigen. Normal oral mucosa showed no expression of these blood group antigens. Surprisingly, Lewis y antigen disappeared in the invasion sites where Ki-67 was definitely stained. Over-expression of Lewis y with manipulation of a fucosyltransferase cDNA resulted in suppression of cell growth and invasion, and knockdown of Lewis y also brought about increased cell growth and invasion. In either situations, no changes in the expression of sialyl-Lewis x could be found. Lowered tumor growth and invasion into surrounding tissues were also shown in Lewis y-positive SCC grafts in nu/nu mice. All these results together with alternative staining between Lewis y and Ki-67 in cancer tissues and FUT1 transfectants suggested that loss of Lewis y is a crucial event for the late stage of SCCs.
Volume 30(6)
Pages 585-97
Published 2013-8-1
DOI 10.1007/s10719-012-9458-2
PMID 23242548
MeSH Animals Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / immunology Carcinoma, Squamous Cell / metabolism* Cell Line, Tumor Fucosyltransferases / genetics Fucosyltransferases / metabolism Gene Expression Regulation, Neoplastic Humans Ki-67 Antigen / genetics Ki-67 Antigen / metabolism Lewis Blood Group Antigens / genetics Lewis Blood Group Antigens / metabolism* Mice Mice, Inbred BALB C Mouth Mucosa / metabolism Mouth Mucosa / pathology Mouth Neoplasms / genetics Mouth Neoplasms / immunology Mouth Neoplasms / metabolism* Neoplasm Invasiveness Neoplasm Transplantation Oligosaccharides / genetics Oligosaccharides / metabolism* Organ Specificity Sialyl Lewis X Antigen
IF 2.197
Human and Animal Cells Ca9-22(RCB1976) HSC-3(RCB1975) HSC-2(RCB1945) HSC-4(RCB1902) Sa3(RCB0980) SCCKN(RCB0441)