| RRC ID |
61788
|
| Author |
Hotta H, Hamamura K, Yamashita K, Shibuya H, Tokuda N, Hashimoto N, Furukawa K, Yamamoto N, Hattori H, Toyokuni S, Ueda M, Furukawa K.
|
| Title |
Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.
|
| Journal |
Glycoconj J
|
| Abstract |
Expression and implication of carbohydrate antigens in squamous cell carcinomas (SCCs) in oral cavity was examined. In the cell lines, type 2H and Lewis y antigens were markedly expressed. In the tissues from SCC patients and benign disorders, type 2H was highly expressed in hyperplasia (96.4 %), displasia (92.9 %) and SCC (100 %). Lewis y was, in turn, expressed mainly in cancer tissues (91.3 %), suggesting that Lewis y is a cancer-associated antigen. Normal oral mucosa showed no expression of these blood group antigens. Surprisingly, Lewis y antigen disappeared in the invasion sites where Ki-67 was definitely stained. Over-expression of Lewis y with manipulation of a fucosyltransferase cDNA resulted in suppression of cell growth and invasion, and knockdown of Lewis y also brought about increased cell growth and invasion. In either situations, no changes in the expression of sialyl-Lewis x could be found. Lowered tumor growth and invasion into surrounding tissues were also shown in Lewis y-positive SCC grafts in nu/nu mice. All these results together with alternative staining between Lewis y and Ki-67 in cancer tissues and FUT1 transfectants suggested that loss of Lewis y is a crucial event for the late stage of SCCs.
|
| Volume |
30(6)
|
| Pages |
585-97
|
| Published |
2013-8-1
|
| DOI |
10.1007/s10719-012-9458-2
|
| PMID |
23242548
|
| MeSH |
Animals
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / immunology
Carcinoma, Squamous Cell / metabolism*
Cell Line, Tumor
Fucosyltransferases / genetics
Fucosyltransferases / metabolism
Gene Expression Regulation, Neoplastic
Humans
Ki-67 Antigen / genetics
Ki-67 Antigen / metabolism
Lewis Blood Group Antigens / genetics
Lewis Blood Group Antigens / metabolism*
Mice
Mice, Inbred BALB C
Mouth Mucosa / metabolism
Mouth Mucosa / pathology
Mouth Neoplasms / genetics
Mouth Neoplasms / immunology
Mouth Neoplasms / metabolism*
Neoplasm Invasiveness
Neoplasm Transplantation
Oligosaccharides / genetics
Oligosaccharides / metabolism*
Organ Specificity
Sialyl Lewis X Antigen
|
| IF |
2.197
|
| Resource |
| Human and Animal Cells |
Ca9-22(RCB1976)
HSC-3(RCB1975)
HSC-2(RCB1945)
HSC-4(RCB1902)
Sa3(RCB0980)
SCCKN(RCB0441) |
