Reference - Detail
| RRC ID | 61805 |
|---|---|
| Author | Katsuyama H, Saijoh K, Otsuki T, Tomita M, Fukunaga M, Sunami S. |
| Title | Menaquinone-7 regulates gene expression in osteoblastic MC3T3E1 cells. |
| Journal | Int J Mol Med |
| Abstract |
Previous study has shown that the vitamin K2 analog menaquinone-7 (MK-7) induces expression of the osteoblast-specific genes osteocalcin, osteoprotegerin, receptor activator of NFkappaB, and its ligand. Since MK-7 may also regulate osteoblast cell function, we examined the expression of osteoblast genes regulated by MK-7 administration. Differences between gene expression in control and MK-7-administered MC3T3E1 cells were analyzed using the suppression subtractive hybridization method. After 24 h of MK-7 administration, genes upregulated by MK-7 included tenascin C and BMP2. Genes downregulated by MK-7 administration included biglycan and butyrophilin. Real-time PCR showed a marked increase in tenascin C. When the protein level was examined using Western blot analysis, tenascin C was higher in MK-7-administered cells than in control cells. These results indicated that MK-7 affected the cellular function of osteoblastic MC3T3E1 cells. Considering BMP2 mRNA expression was higher in MK-7-administered cells than in control cells, the effect of MK-7 administration on the signal transduction system was examined. Western blot analysis showed that cells administered MK-7 displayed a higher phosphorylated Smad1 level than control cells. Because MC3T3E1 cells have a nuclear binding receptor for MK-7, this result might indicate an indirect effect of MK-7 through BMP2 production. |
| Volume | 19(2) |
| Pages | 279-84 |
| Published | 2007-2-1 |
| PMID | 17203202 |
| MeSH | Animals Cell Line Gene Expression Regulation / drug effects* Mice Nucleic Acid Hybridization Osteoblasts / drug effects* Osteoblasts / metabolism* RNA, Messenger / genetics Signal Transduction Tenascin / genetics Vitamin K 2 / analogs & derivatives* Vitamin K 2 / pharmacology |
| IF | 3.098 |
| Altmetric score |
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| The most frequently cited source | |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |