| RRC ID |
61853
|
| 著者 |
Dalai W, Matsuo E, Takeyama N, Kawano J, Saeki K.
|
| タイトル |
Increased expression of prion protein gene is accompanied by demethylation of CpG sites in a mouse embryonal carcinoma cell line, P19C6.
|
| ジャーナル |
J Vet Med Sci
|
| Abstract |
Elucidation of the processes regulating the prion protein gene (Prnp) is an important key to understanding the development of prion disorders. In this study, we explored the involvement of DNA methylation in Prnp transcriptional regulation during neuronal differentiation of embryonic carcinoma P19C6 cells. When P19C6 cells were differentiated into neuronal cells, the expression of Prnp was markedly increased, while CpG methylation was significantly demethylated at the nucleotide region between -599 and -238 from the transcription start site. In addition, when P19C6 cells were applied in a DNA methyltransferase inhibitor, RG108, Prnp transcripts were also significantly increased in relation to the decreased methylation statuses. These findings helped to elucidate the DNA methylation-mediated regulation of Prnp expression during neuronal differentiation.
|
| 巻・号 |
79(3)
|
| ページ |
644-648
|
| 公開日 |
2017-3-23
|
| DOI |
10.1292/jvms.16-0554
|
| PMID |
28132962
|
| PMC |
PMC5383191
|
| MeSH |
Animals
Cell Differentiation
Cell Line
CpG Islands
DNA Methylation*
Epigenesis, Genetic
Gene Expression Regulation
Mice
Neurons / cytology
Neurons / metabolism
Prion Proteins / genetics*
Prions / genetics
Transcription, Genetic
|
| IF |
1.049
|
| リソース情報 |
| ヒト・動物細胞 |
P19C6 |
