Reference - Detail
| RRC ID | 61881 |
|---|---|
| Author | Duhart JM, Herrero A, de la Cruz G, Ispizua JI, Pírez N, Ceriani MF. |
| Title | Circadian Structural Plasticity Drives Remodeling of E Cell Output. |
| Journal | Curr Biol |
| Abstract |
Behavioral outputs arise as a result of highly regulated yet flexible communication among neurons. The Drosophila circadian network includes 150 neurons that dictate the temporal organization of locomotor activity; under light-dark (LD) conditions, flies display a robust bimodal pattern. The pigment-dispersing factor (PDF)-positive small ventral lateral neurons (sLNv) have been linked to the generation of the morning activity peak (the "M cells"), whereas the Cryptochrome (CRY)-positive dorsal lateral neurons (LNds) and the PDF-negative sLNv are necessary for the evening activity peak (the "E cells") [1, 2]. While each group directly controls locomotor output pathways [3], an interplay between them along with a third dorsal cluster (the DN1ps) is necessary for the correct timing of each peak and for adjusting behavior to changes in the environment [4-7]. M cells set the phase of roughly half of the circadian neurons (including the E cells) through PDF [5, 8-10]. Here, we show the existence of synaptic input provided by the evening oscillator onto the M cells. Both structural and functional approaches revealed that E-to-M cell connectivity changes across the day, with higher excitatory input taking place before the day-to-night transition. We identified two different neurotransmitters, acetylcholine and glutamate, released by E cells that are relevant for robust circadian output. Indeed, we show that acetylcholine is responsible for the excitatory input from E cells to M cells, which show preferential responsiveness to acetylcholine during the evening. Our findings provide evidence of an excitatory feedback between circadian clusters and unveil an important plastic remodeling of the E cells' synaptic connections. |
| Volume | 30(24) |
| Pages | 5040-5048.e5 |
| Published | 2020-12-21 |
| DOI | 10.1016/j.cub.2020.09.057 |
| PII | S0960-9822(20)31427-5 |
| PMID | 33065014 |
| MeSH | Acetylcholine / metabolism Animals Animals, Genetically Modified Biological Clocks / physiology* Circadian Rhythm / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / physiology* Feedback, Physiological Glutamic Acid / metabolism Locomotion / physiology* Male Period Circadian Proteins / genetics Period Circadian Proteins / metabolism Photoperiod Presynaptic Terminals / metabolism* |
| IF | 9.601 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 18 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 5549R-2 |