RRC ID 61882
Author Chakrabarti S, Visweswariah SS.
Title Intramacrophage ROS Primes the Innate Immune System via JAK/STAT and Toll Activation.
Journal Cell Rep
Abstract Tissue injury is one of the most severe environmental perturbations for a living organism. When damage occurs in adult Drosophila, there is a local response of the injured tissue and a coordinated action across different tissues to help the organism overcome the deleterious effect of an injury. We show a change in the transcriptome of hemocytes at the site of tissue injury, with pronounced activation of the Toll signaling pathway. We find that induction of the cytokine upd-3 and Toll receptor activation occur in response to injury alone, in the absence of a pathogen. Intracellular accumulation of hydrogen peroxide in hemocytes is essential for upd-3 induction and is facilitated by the diffusion of hydrogen peroxide through a channel protein Prip. Importantly, hemocyte activation and production of reactive oxygen species (ROS) at the site of a sterile injury provide protection to flies on subsequent infection, demonstrating training of the innate immune system.
Volume 33(6)
Pages 108368
Published 2020-11-10
DOI 10.1016/j.celrep.2020.108368
PII S2211-1247(20)31357-7
PMID 33176146
PMC PMC7662148
MeSH Animals Drosophila / metabolism* Drosophila Proteins / metabolism* Immunity, Innate / physiology* Janus Kinases / metabolism* Macrophages / metabolism* Reactive Oxygen Species STAT Transcription Factors / metabolism*
IF 8.109
Drosophila 5988R-3,7873R-3