RRC ID 61884
Author Huynh N, Depner N, Larson R, King-Jones K.
Title A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila.
Journal Genome Biol
Abstract Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 different Cas13 variants to identify enzymes with the best overall performance and show that Cas13 could target endogenous Drosophila transcripts in vivo with high efficiency and specificity. We also develop Cas13 applications to edit mRNAs and target mitochondrial transcripts. Our vector collection represents a versatile tool collection to manipulate gene expression at the post-transcriptional level.
Volume 21(1)
Pages 279
Published 2020-11-17
DOI 10.1186/s13059-020-02193-y
PII 10.1186/s13059-020-02193-y
PMID 33203452
PMC PMC7670108
MeSH Adenosine Deaminase / metabolism Animals CRISPR-Associated Proteins / metabolism CRISPR-Cas Systems* Drosophila / genetics* Endonucleases / metabolism Gene Expression RNA / genetics* RNA Processing, Post-Transcriptional* RNA, Mitochondrial RNA-Binding Proteins / metabolism
IF 10.806
Drosophila TBX-0010