| RRC ID |
61890
|
| 著者 |
Chang PJ, Wang SS, Chen LY, Hung CH, Huang HY, Shih YJ, Yen JB, Liou JY, Chen LW.
|
| タイトル |
ORF50-dependent and ORF50-independent activation of the ORF45 gene of Kaposi's sarcoma-associated herpesvirus.
|
| ジャーナル |
Virology
|
| Abstract |
The ORF45 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a multifunctional tegument protein. Here, we characterize the transcriptional control of the ORF45 gene and show that its promoter can be activated by ORF50 protein, a latent-lytic switch transactivator. The ORF45 promoter can also be induced by sodium butyrate (SB), a histone deacetylase inhibitor, in the absence of ORF50 protein. Although SB induces the ORF45 gene independently of ORF50, its full activation may require the presence of ORF50. Deletion and point mutation analyses revealed that two RBP-Jκ-binding sites in the ORF45 promoter confer the ORF50 responsiveness, whereas NF-Y and Sp1-binding sites mediate the response to SB. Direct binding of NF-Y, Sp1, or RBP-Jκ protein to the ORF45 promoter is required for the promoter activation induced by SB or by ORF50. In conclusion, our study demonstrates both ORF50-dependent and ORF50-independent transcriptional mechanisms operated on the activation of the ORF45 gene.
|
| 巻・号 |
442(1)
|
| ページ |
38-50
|
| 公開日 |
2013-7-20
|
| DOI |
10.1016/j.virol.2013.03.023
|
| PII |
S0042-6822(13)00176-1
|
| PMID |
23601787
|
| MeSH |
Binding Sites
Butyrates / metabolism
Butyrates / pharmacology
CCAAT-Binding Factor
Gene Expression Regulation, Viral*
Herpesvirus 8, Human / genetics
Herpesvirus 8, Human / metabolism
Herpesvirus 8, Human / physiology*
Humans
Immediate-Early Proteins / genetics
Immediate-Early Proteins / metabolism*
Immunoglobulin J Recombination Signal Sequence-Binding Protein / genetics
Immunoglobulin J Recombination Signal Sequence-Binding Protein / metabolism
Protein Kinases
Trans-Activators / genetics
Trans-Activators / metabolism*
Viral Proteins / genetics
Viral Proteins / metabolism
Virus Activation*
|
| IF |
2.819
|
| リソース情報 |
| ヒト・動物細胞 |
OT11(RCB1926)
OT13(RCB1927) |
