論文 - 詳細
| RRC ID | 61924 |
|---|---|
| 著者 | Sumaru K, Takagi T, Morishita K, Kanamori T. |
| タイトル | Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition. |
| ジャーナル | Biomacromolecules |
| Abstract |
We report a sharp photoinduced aqueous dissolution of the copolymer through phase transition based on the photochemical reaction of o-nitrobenzaldehyde (NBA) and the principle of polymer effect. We synthesized the copolymers having poly( N-isopropylacrylamide) main chain and NBA side chain at 4, 7, and 10 mol % functionalizations and analyzed their photoresponsive characteristics. Light with 365 nm wavelength converted NBA groups at copolymer side chains to carboxylic acid efficiently at the rate of 7.3 cm2/J, and in the case of 10 mol % functionalization, the irradiation dosage no more than 56 mJ/cm2 induced sharp aqueous dissolution of the copolymer thin layer in pH 7.4 at 25 °C. As example applications, we demonstrated on-demand release of polyethylene beads and fluorescent-labeled albumins, which had been immobilized on a substrate surface via the copolymers, by the precisely controlled light irradiation using a microprojection system. Also, we examined application of the copolymers to the selective recovery of living cells from culture substrate under microscopic observation. As a result, mild light irradiation at room temperature triggered immediate detachment of the cultured adherent cells only in the irradiated areas without critical influence on their viability. |
| 巻・号 | 19(7) |
| ページ | 2913-2922 |
| 公開日 | 2018-7-9 |
| DOI | 10.1021/acs.biomac.8b00470 |
| PMID | 29641886 |
| MeSH | 3T3 Cells Acrylic Resins / chemistry* Albumins / chemistry Animals Benzaldehydes / chemistry* Dogs Fluorescent Dyes / chemistry HeLa Cells Humans Light* Madin Darby Canine Kidney Cells Mice Phase Transition* Photochemical Processes* |
| IF | 6.092 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) MDCK(RCB0995) |