論文 - 詳細
| RRC ID | 61949 |
|---|---|
| 著者 | Xu HH, Carey LE, Simon CG Jr, Takagi S, Chow LC. |
| タイトル | Premixed calcium phosphate cements: synthesis, physical properties, and cell cytotoxicity. |
| ジャーナル | Dent Mater |
| Abstract |
OBJECTIVES:Calcium phosphate cement (CPC) is a promising material for dental, periodontal, and craniofacial repairs. However, its use requires on-site powder-liquid mixing that increases the surgical placement time and raises concerns of insufficient and inhomogeneous mixing. The objective of this study was to determine a formulation of premixed CPC (PCPC) with rapid setting, high strength, and good in vitro cell viability. METHODS:PCPCs were formulated from CPC powder+non-aqueous liquid+gelling agent+hardening accelerator. Five PCPCs were thus developed: PCPC-Tartaric, PCPC-Malonic, PCPC-Citric, PCPC-Glycolic, and PCPC-Malic. Formulations and controls were compared for setting time, diametral tensile strength, and osteoblast cell compatibility. RESULTS:Setting time (mean+/-S.D.; n=4) for PCPC-Tartaric was 8.2+/-0.8 min, significantly less than the 61.7+/-1.5 min for the Premixed Control developed previously (p<0.001). On 7th day immersion, the diametral tensile strength of PCPC-Tartaric reached 6.5+/-0.8 MPa, higher than 4.5+/-0.8 MPa of Premixed Control (p=0.036). Osteoblast cells displayed a polygonal morphology and attached to the nano-hydroxyapatite crystals in the PCPCs. All cements had similar live cell density values (p=0.126), indicating that the new PCPCs were as cell compatible as a non-premixed CPC control known to be biocompatible. Each of the new PCPCs had a cell viability that was not significantly different (p>0.1) from that of the non-premixed CPC control. SIGNIFICANCE:PCPCs will eliminate the powder-liquid mixing during surgery and may also improve the cement performance. The new PCPCs supported cell attachment and yielded a high cell density and viability. Their mechanical strengths approached the reported strengths of sintered porous hydroxyapatite implants and cancellous bone. These nano-crystalline hydroxyapatite cements may be useful in dental, periodontal, and craniofacial repairs. |
| 巻・号 | 23(4) |
| ページ | 433-41 |
| 公開日 | 2007-4-1 |
| DOI | 10.1016/j.dental.2006.02.014 |
| PII | S0109-5641(06)00066-2 |
| PMID | 16678895 |
| PMC | PMC2646467 |
| MeSH | 3T3 Cells Animals Calcium Phosphates / toxicity* Cell Adhesion Cell Survival Dental Cements / chemical synthesis Dental Cements / toxicity* Dental Stress Analysis Durapatite Hydroxyapatites / chemical synthesis Hydroxyapatites / toxicity* Mice Nanostructures Osteoblasts / drug effects* Tartrates / chemistry Tensile Strength |
| IF | 4.495 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |